HPLC法测定复方硫唑乳膏中甲硝唑含量

目的建立高效液相色谱法测定复方硫唑乳膏中甲硝唑的含量。方法采用Agilent C18柱(4.6 mm×250mm,5μm),以甲醇-水(20∶80)为流动相,流速1 mL·min-1,检测波长320 nm,柱温30℃。结果甲硝唑进样量在0.809 3～5.665 1μg范围内呈良好线性关系(r=1),平均回收率100.7%,RSD=1.3%。结论本方法结果准确,专属性强,重现性好,可作为复方硫唑乳膏中甲硝唑的含量测定方法。

Determination of metronidazole in Compound Sublimed Sulfur and Metronidazole Cream by HPLC

Objective To establish an HPLC method for the determination of metronidazole in Compound Sublimed Sulfur and Metronidazole Cream. Methods Agilent C ls column （ 4.6 mm ×250 mm,5 μm ）was adopted with methanol -water（20： 80） as the mobile phase. The flow rate was 1.0 mL·min-1 ,the detection wavelength was 320 nm and the column temperature was 30 ℃. Results Metronidazole showed a good linear relationship within 0. 809 3 - 5. 665 1 μg （ r = 1 ）. The average recovery was 100.7% and the RSD was 1.3%. Conclusion The method was accurate with good specificity and reproducibility, and it was suitable for the determination of metronidazole in Compound Sublimed SuLfur and Metronidazole Cream.