| 酶联免疫吸附法检测HBsAg的假阳性和解决方法 |
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| 引用本文： | 杨寿旺,李睿,黄建可.酶联免疫吸附法检测HBsAg的假阳性和解决方法[J].中国国境卫生检疫杂志,2006,29(5):273-274. |
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| 作者姓名： | 杨寿旺 李睿 黄建可 |
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| 作者单位： | 温州国际旅行卫生保健中心,温州,325000 |
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| 基金项目： | 致谢：感谢温州医学院附属第二医院检验科的大力支持! |
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| 摘 要： | 〔目的〕避免酶联免疫吸附试验(ELISA)检测HBsAg假阳性报告的发生。〔方法〕对ELISA法检测HBsAg结果为阳性而金标法为阴性的69份血液标本,用ELISA法和Abbott免疫发光法检测乙型肝炎3系5项免疫指标,并对结果进行分析。〔结果〕ELISA法检测HBsAg的假阳性率为1.19%,而用同样方法复检后,其假阳性率降至0.06%。〔结论〕ELISA法检测HBsAg的假阳性大多可通过用同样方法复检并结合乙型肝炎3系的其他免疫指标发现并给予纠正,对少数复检结果仍难以确定者,可用Abbott免疫发光法检测乙肝3系5项免疫指标给予确认。
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| 关 键 词： | 肝炎表面抗原 乙型 酶联免疫吸附法 假阳性 弱阳性 |
| 收稿时间： | 2006-01-20 |
| 修稿时间： | 2006年1月20日 |
The False Positive of HBsAg Detected by Enzyme-linked Immunosorbent Assay (ELISA) and the Solution |
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| Yang Shouwang,Li Rui,Huang Jianke.The False Positive of HBsAg Detected by Enzyme-linked Immunosorbent Assay (ELISA) and the Solution[J].Chinese Journal of Frontier Health and Quarantine,2006,29(5):273-274. |
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| Authors: | Yang Shouwang Li Rui Huang Jianke |
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| Institution: | Wenzhou International Travel Health Care center,Wenzhou 325000,China |
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| Abstract: | Objective To avoid the false positive of HBsAg detected by enzyme-linked immunosorbent assay(ELISA).Methods The 69 blood specimens of which HBsAg were positive detected by ELISA and negative by immunogold histochemistry technique were selected.Their HBsAg,HBsAb,HBeAg,HBeAb and HBcAb were detected by ELISA and Abbott luminescence immunoassay repeatedly,then the results were analyzed.Results The ratio of false positive of HBsAg detected by ELISA was 1.19%,however it reduced to 0.06% after detected by ELISA again.Conclusion Most of the false positive of HBsAg detected by ELISA could be corrected by ELISA again,and combine with the analysis of their HBsAg,HBsAb,HBeAg,HBeAb and HBcAb.Some uncertain results could be confirmed by Abbott luminescence immunoassay. |
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| Keywords: | HBsAg ELISA False positive Weak positive |
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