银屑病皮损和正常人皮肤五种细胞外基质成分的免疫组化检测

用 ＰＡＰ法显示１８例银屑病皮损和 ７例健康人皮肤Ⅰ、Ⅲ、Ⅳ 型胶原蛋白（简写为Ⅰｃ、Ⅲｃ、Ⅳｃ）、纤维连接蛋白（ＦＮ）和基膜连接蛋白（ＬＮ）的分布。在正常人皮肤中 Ｉｃ和Ⅲｃ分布于真皮层，而在银屑病皮损中Ｉｃ、Ⅲｃ显色较正常人皮肤深，在真皮浅层、血管周围及炎细胞浸润区呈局灶性浅染。正常人皮肤中Ⅳｃ、 ＦＮ和 ＬＮ呈线状分布于基底膜，在银屑病皮损中则呈不规则节段样增粗和缺损； ＦＮ和ＬＮ在乳头顶端明显缺损，其两边呈网状增厚。此结果可能与成纤维细胞和角朊细胞的合成异常增强有关； Ｉｃ和Ⅲｃ的区域性浅染及基底膜成分在乳头顶端的缺损则可能与炎症细胞的吞噬及释放的蛋白水解酶的破坏有关。

Immunohistochemical Study of Collagen , Fibronectin and Laminin in Skin Lesions of Patients with Psoriasis

The distribution of collagen fibronectin (FN) and laminin (LN) was studied in 18 skin lesions of patients with psoriasis and 7 normal human skin by PAP method. The Lc and c were present in the dermis of normal skin. In psoriatic lesions, the I c and c stained darker than those in normal skin, and stained lightly in focal areas of upper dermis, periphery of blood vessels and regions with infiltration. The c, FN and LN distributed linearly in the basement membrane (BM), while they showed irregular thickness with segmental defects in the psoriatic epidermal BM. FN and LN were obviously absent in the top of the papillae, whereas they were net-like thickened on the two sides of the top of the papiliae. These results might be related to the increased synthesis of the fibroblasts and keratinocytes in the psoriatic lesions, but the focal light stain of I c and c and the segmental defects of the components of the BM might be related to the phagocytosis of the inflammatory cells and the destruction of proteolytic enzyme released.