未动员的外周血造血干细胞采集效果及影响因素

目的探讨未动员的外周血造血干细胞(PBSC)采集效果及影响因素。方法应用血细胞分离机对112例未经重组人粒细胞集落刺激因子(rhG-CSF)动员的健康供者进行PBSC采集,并分析年龄、体质量指数(BMI)、采集前血常规指标、采集循环血容量、处理血量及循环次数等因素对男女两组供者所采集获得的单个核细胞(MNC)、CD34+计数的影响,同时比较分析男女两组供者采集前血常规指标及采集过程、采集物等指标。结果男性组年龄,BMI,采集前血细胞比容(Hct)、血红蛋白(Hb)、MNC计数、白细胞(WBC)计数,总循环血量高于女性组,而采集处理血量、采集物中MNC计数低于女性组,差异均有统计学意义(P<0.05)。在男性组中,采集物中MNC计数的影响因素为采集循环数(P=0.018),CD34+计数的影响因素为采集前血小板(PLT)计数(P=0.048)。女性组年龄、采集前PLT计数、WBC计数、MNC百分比、MNC计数是采集物中MNC计数的影响因素(P<0.05),采集前Hct、PLT计数及采集处理血量是采集物中CD34+计数的影响因素(P<0.05)。结论未经rhG-CSF动员的健康供者其外周血中存在一定数量的MNC、CD34+细胞,并能采集到满足临床嵌合抗原受体T细胞疗法所需要的MNC阈值。健康供者不同年龄、性别、总循环血量等可致采集效果不一致;采集前关注血常规中PLT计数有助于预测PBSC采集效果。

Influenced factors on the collection of peripheral blood stem cells without mobilization in healthy donors

Objective To investigate the factors influencing the efficiency of the collection of peripheral blood stem cells(PBSC)without mobilization.Methods The records of 112 cases with healthy donors without mobilization of recombinant human granulocyte colony stimulating factor(rhG-CSF)were retrospectively analyzed.PBSC were collected by MCS+blood cells separator.The influences of age,body mass index(BMI),blood routine index indicators before collection,circulating blood volume,blood volume and number of cycles on the mononuclear cells(MNC)and CD34+in the collection between male group and female group were analyzed.Blood routine indicators,collection process and collected objects were also compared between male group and female group.Results The age,BMI,pre-collection hematocrit(Hct),hemoglobin(Hb),MNC count WBC count and total circulation of the male group were significantly higher than those of the female group(P<0.05),while processed blood volume and MNC counts were significantly lower than that of female group(P<0.05).The number of collection cycles was the influenced factors of the MNC count of the male group(P=0.018),and the CD34+counts was the influenced factor of PLT count before collection(P=0.048).Age,PLT count before collection,the WBC count and the percent of MNC and MNC count were the influenced factors of MNC count in the collection in female group(P<0.05).Hct,PLT count and processed blood volume were the influenced factors of CD34+count in the collection(P<0.05).Conclusion Donors who are not mobilized have a certain number of MNC and CD34+cells in their peripheral blood,which could collect the MNC threshold required to meet clinical CAR-T cells therapy.Different age,gender,the total circulation the amount of blood circulation may result in inconsistent collection results.Focusing on the PLT count in blood routines before collection could help predict the acquisition effect of PBSC.