不同类型标本及仪器对血糖检测结果的影响

目的探讨罗氏血糖仪与全自动生化分析仪检测不同类型标本血糖水平的差异。方法收集2017年2-5月于南京市栖霞区妇幼保健院就诊患者及体检者的末梢血及静脉血标本104例。比较罗氏血糖仪检测的末梢血(末梢血POCT组)、静脉全血(全血POCT组)、静脉血浆(血浆POCT组)、静脉血清(血清POCT组)和全自动生化分析仪检测的静脉血浆(血浆生化仪组)、静脉血清(血清生化仪组)的血糖水平;验证罗氏血糖仪的精密度及线性范围。结果3台罗氏血糖仪的批内CV和批间CV均小于7.5%,符合判定标准。罗氏血糖仪检测的静脉全血血糖水平在1.1~24.4 mmol/L呈线性,R 2>0.95;静脉血清血糖水平在0.6~25.3 mmol/L呈线性,R 2>0.95,均符合线性要求。血糖水平<6 mmol/L的检测结果中,全血POCT组血糖水平低于其余各组(P<0.05);血糖水平为6~10 mmol/L的检测结果中,全血POCT组血糖水平低于血浆POCT组、血清POCT组、血浆生化仪组及血清生化仪组(P<0.05)。各组检测的血糖水平两两之间均具有显著相关性(r>0.99,P<0.05)。结论罗氏血糖仪检测精密度高,具有良好的线性,末梢血、静脉血浆、静脉血清等标本在血糖仪上的检测结果与全自动生化分析仪检测结果具有较好的可比性;罗氏血糖仪检测静脉全血时需进行一定程度的校正以保证结果的准确性。

Effects of different types of specimens and instruments on the result of blood glucose

Objective To explore the difference in detecting the blood glucose(Glu)of different specimens between the Roche blood glucose meter and automatic biochemical analyzer.Methods The peripheral blood and venous blood specimens of 104 patients admitted to Maternal and Child Health Hospital of Qixia District from February to May 2017 were collected.The Glu levels of peripheral blood(peripheral blood POCT group),venous whole blood(whole blood POCT group),venous plasma(plasma POCT group),venous serum(serum POCT group)detected by the Roche blood glucose meter,venous plasma(plasma biochemical analyzer group)and venous serum(serum biochemical analyzer group)detected by automatic biochemical analyzer were compared.The precision and linear range of the Roche blood glucose meter were verified.Results The intra and inter CV of the 3 Roche blood glucose meters were less than 7.5%,which met the criteria.The venous whole Glu level detected by the Roche blood glucose meter was linear in the range of 1.1 to 24.4 mmol/L,and R 2>0.95;the venous serum Glu level was linear in the range of 0.6 to 25.3 mmol/L,and R 2>0.95,all met the linear requirements.In the Glu level<6 mmol/L,the Glu level of the whole blood POCT group was lower than that in other groups(P<0.05);in the Glu level of 6 to 10 mmol/L,the Glu level of the whole blood POCT group was lower than that in the plasma POCT group,serum POCT group,plasma biochemical analyzer group and serum biochemical analyzer group(P<0.05).There were significant correlation between Glu levels in each group(r>0.99,P<0.05).Conclusion The detection accuracy of Roche blood glucose meter is high,and it has good linearity.The detection results of peripheral blood,venous plasma,venous serum on the Roche blood glucose meter are comparable with those of the automatic biochemical analyzer.The detection results of venous whole blood on the Roche blood glucose meter need to be corrected to a certain extent to ensure the accuracy of the results.