2144例儿童11项呼吸道病原体IgM抗体检测结果分析

目的探讨间接免疫荧光法(IIF)检测儿童血清11项呼吸道病原体免疫球蛋白M抗体(IgM抗体)的分布及其与年龄、季节、感染类型的关系。方法选择2017年8月至2018年7月在自贡市第一人民医院采用IIF进行11项呼吸道病原体IgM抗体检测的2144例儿童患者结果进行回顾性分析。结果2144例儿童采用IIF检测11项呼吸道病原体IgM抗体,总阳性率为47.7%(1023/2144);其中阳性率较高的分别是流感病毒B(INFB,34.8%)、流感病毒A(INFA,17.9%)、嗜肺军团菌(LP,7.6%)、肺炎支原体(MP,6.7%);不同年龄段0~<4岁、4~<7岁、7~<14岁间阳性率比较,后两组高于0~<4岁组,差异有统计学意义(P<0.05),其中不同年龄段间呼吸道合胞病毒(RSV)、INFA、INFB、肺炎衣原体(CP)、LP、MP的IgM抗体的阳性率比较,差异有统计学意义(P<0.05),但不同年龄段间感染类型(单一感染、多重感染)比较,差异无统计学意义(P>0.05);儿童在秋、冬季节血清11项呼吸道病原体IgM抗体阳性率明显高于春、夏季,差异有统计学意义(P<0.05),但不同季节间感染类型(单一感染、多重感染)比较,差异无统计学意义(P>0.05)。结论儿童11项呼吸道病原体检测结果在不同年龄、季节有一定差异性。

Analysis of 11 respiratory pathogen IgM antibody detected by indirect immunofluorescence in 2144 cases of children

Objective To investigate the distribution of immunoglobulin M antibody(IgM antibodies)to 11 respiratory pathogens detected by indirect immunofluorescence in children serum and its relationship with age,season and type of infection.Methods 2144 cases of children with 11 respiratory pathogens were selected from August 2017 to July 2018 in First People′s Hospital of Zigong.Results The total positive rate of IgM antibodies against 11 respiratory pathogens was 47.7%(1023/2144)in 2144 children by indirect immunofluorescence assay.The higher positive rates were INFB(34.8%),INFA(17.9%),LP(7.6%)and MP(6.7%);In different age groups,the positive rates of 4-<7 years old group and 7-<14 years old group were higher than 0-<4 years old group.The difference was statistically significant(P<0.05).The positive rates of IgM antibodies of RSV,INFA,INFB,CP,LP and MP in different age groups were significantly different(P<0.05),but there was no significant difference in infection types(single infection and multiple infection)between different age groups(P>0.05).The positive rate of IgM antibody of 11 respiratory pathogens in children′s serum in autumn and winter was significantly higher than that in spring and summer,and the difference was statistically significant(P<0.05).But there was no significant difference in infection types(single infection,multiple infection)between different seasons(P>0.05).Conclusion The results of 11 respiratory pathogens in children are different in different ages and seasons.