HCO3^-在不同检测仪器中测定结果的相关性及在静脉血中的稳定性分析

目的探讨血气分析仪与生化分析仪检测碳酸氢根离子(HCO3^-)结果的可比性,以及标本放置时间对血清中HCO3^-检测结果的影响。方法分别用GM3000血气分析仪与VITROS4600生化分析仪对70例患者的动脉全血和静脉血清标本进行检测,比较两种仪器检测的HCO3&-结果是否具有差异性及相关性。随机收集70例患者中的20份静脉血标本,分别于4个不同时间点(30 min、60 min、120 min和240 min)检测血清标本中HCO3^-水平,评估标本放置时间对HCO3^-检测结果的影响。结果VITROS4600生化分析仪与GM3000血气分析仪检测的HCO3^-水平比较,差异无统计学意义(P>0.05)。相关性分析结果显示,两台仪器检测结果之间具有良好的相关性(r=0.956,P<0.05)。与血清标本在室温下保存30 min时HCO3^-的检测结果比较,标本在室温下保存60 min时,检测结果下降了5.1%,保存120 min时,结果下降了8.8%,保存240 min时,结果下降了10.0%。结论GM3000血气分析仪与VITROS 4600生化分析仪检测的HCO3^-水平无明显差异,呈良好的相关性,两种仪器检测的HCO3^-结果可互相替代。随着标本放置时间的延长,血清中HCO3^-水平不断下降。

Correlation of bicarbonate detection results among different detection instruments and its stability in venous blood

Objective To investigate the comparability between the blood gas analyzer and biochemical analyzer for detecting HCO3^- level and the influence of the sample place time on serum HCO3^- detection results.Methods The arterial whole blood and venous serum samples from 70 patients were detected by using the GM3000 blood gas analyzer and VITROS4600 biochemical analyzer.Then whether the HCO3^- detection results possessing the difference and correlation were compared between these two kinds of detection instruments.Twenty venous samples were randomly collected from 70 patients.The HCO3^- levels at 4 different time points(30,60,120,240 min)were detected.Then the influence of the sample place time on the HCO3^- detection results was evaluated.Results The detection results of HCO3^- had no statistical difference between the GM3000 blood gas analyzer and VITROS4600 biochemical analyzer(P>0.05).The correlation analysis results showed that there was a good correlation between the results of two kinds of instruments(r=0.956,P<0.05).Compared with the HCO3^- detection results for the serum sample in placement for 30 min under the room temperature,the detection results in placement for 60 min under the room temperature was decreased by 5.1%,which in placement for 120 min was decreased by 8.8%and which in placement for 240 min was decreased by 10.0%.Conclusion There was no significant difference in the detection results of HCO3^- by the GM3000 blood gas analyzer and VITROS4600 biochemical analyzer,showing good correlation.The HCO3^- results detected by these two kinds of instruments can be substituted for each other.With the extension of sample placement time,serum HCO3^- level is decreased continuously.