社区肺炎链球菌感染药敏分析及分子生物学分型

目的调查该院肺炎链球菌药敏谱及耐药基因型,为肺炎链球菌感染的预防与治疗提供参考。方法收集该院2014年1月至2016年12月分离的肺炎链球菌共184株,用Vitek 2鉴定菌株,检测耐药模式,PCR方法检测耐药基因erm(B)、mef(A)。结果肺炎链球菌对青霉素G的耐药率为60.6%,对红霉素、克林霉素的耐药率分别为93.3%、100.0%。耐药表型为MLSB型的检出率为93.5%(cMLSB:89.7%,iMLSB:3.8%),未检出M耐药表型。耐药基因erm(B)的检出率为91.3%,mef(A)的检出率为1.6%。结论该院肺炎链球菌对红霉素与克林霉素的耐药率较高;耐药表型以erm(B)编码的cMLSB耐药表型为主,主要以靶位点的修饰为主,也与泵外排机制有关。

Antibiotic sensitivity spectrum and molecular epidemiology among Streptococcus Pneumoniae from community hospital

Objective To determine the antibiotic sensitivity spectrum and resistant phenotypes of Streptococcus Pneumoniae in Xiaolan Hospital Affiliated to Southern Medical University order to provide a reasonable guidance for prevention and treatment.Methods A total of 184 Streptococcus Pneumoniae strains were collected from January 2014 to December 2016.Vitek 2 was used to identify the isolates and the resistant phenotypes.PCR was used to detect the resistant genes erm(B)and mef(A)genes.Results Penicilin G resistance was found in 60.6% of Streptococcus Pneumoniae isolated.The resistant rates of erythromycin,clindamycin were 93.3%,100.0% respectively.The detection rate of the phenotype MLSB was 93.5%(cMLSB:89.7%,iMLSB:3.8%).The phenotype M was not found.The detection rates of erm(B)gene and mef(A)gene were91.3%,1.6%respectively.Conclusion The resistant rates of Streptococcus Pneumoniae to erythromycin and clindamycin are still high.cMLSB mediated by erm(B)gene is the most predominant phenotype among macrolide-resistant Streptococcus Pneumoniae,and it is also mediated by efflux pumps.