利拉鲁肽对急性心肌梗死早期心脏功能的影响研究

目的 探讨利拉鲁肽对急性心肌梗死(AMI)早期心脏功能是否具有保护作用,并阐明其可能机制.方法 45只Wistar大鼠,其中37只通过结扎冠状动脉左前降支制作AMI模型,术后存活16只,随机分为AMI组8只、利拉鲁肽组(Lira组,8只),剩余8只作为假手术组(Sham组,8只),术后24 h给予Lira组利拉鲁肽10...

Effect of liraglutide on cardiac function in the early stage of acute myocardial infarction

Objective To investigate the protective effect of liraglutide on cardiac function in the early stage of acute myocardial infarction(AMI)and clarify its possible mechanism.Methods The AMI model was established by ligating the left anterior descending branch of coronary artery in 37 Wistar rats randomly selected from a total of 45.Sixteen rats survived after operation.They were randomly divided into AMI group(n=8)and liraglutide group(Lira group,n=8).The remaining 8 rats were treated as sham operation group(Sham group,n=8).Twenty-four hours after operation,Lira group was given subcutaneous injection of liraglutide(100μg·kg-1·d-1),while the Sham group and AMI group were given the same amount of normal saline.The experiment was ended at 4 weeks after operation.Then the rats were measured for left ventricular end-diastolic diameter(LVEDd),left ventricular ejection fraction(LVEF)and left ventricular fractional shortening(LVFS)on echocardiography,as well as left ventricular systolic pressure(LVSP),left ventricular end-diastolic pressure(LVEDP),maximum rate of rise of left ventricular pressure(+dp/dt)and maximum rate of decrease of left ventricular pressure(-dp/dt)by hemodynamics study.N-terminal pro basic natriuretic peptide(NT-ProBNP)was measured by ELISA method.Heart weight(HW)and body weight(BW)were measured to calculate heart-body index(HW/BW).Finally,the content of vascular endothelial growth factor(VEGF)protein was detected by Western blotting method.Results After 4 weeks,compared with AMI group,the Lira group showed significant increases in LVEF and LVFS(P<0.05)but not LVEDd between the two groups(P>0.05),higher HW/BW(P<0.01),and remarkable reductions in BW and NT-ProBNP(P<0.01)but not HW(P>0.05);in addition,the Lira group also showed significantly decreased LVEDP(P<0.05),increased+dp/dt and-dp/dt(P<0.05).LVSP did not significantly differ between the two groups(P>0.05).The expression of VEGF protein increased significantly in the Lira group(P<0.01).Conclusion In AMI rats,use of liraglutide leads to reductions in body weight,NT-ProBNP and LVEDP,and increases in LVEF,LVFS,+dp/dt,-dp/dt and VEGF expression.Liraglutide may exhibit cardioprotective effect by reducing volume load and improving microcirculation in the peri-infarcted area in the early stage of AMI.