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双波长双试剂二点法测定血糖的方法研究
引用本文:王克俊,燕东海,陶谦,任更朴,耿海燕. 双波长双试剂二点法测定血糖的方法研究[J]. 实用医技杂志, 2006, 13(1): 32-33
作者姓名:王克俊  燕东海  陶谦  任更朴  耿海燕
作者单位:聊城市第二人民医院,山东,临清,252601
摘    要:目的:建立一种双波长双试剂二点法测定血糖的方法。方法:应用BECKMANCOULTER LX20全自动生化分析仪,用双波长双试剂二点法测定血糖。结果:本法与LX20电极法相关系数r=0.9986,血糖浓度在22.20mmol/L内线性良好,批内CV是2.11%~3.07%,批间CV是3.57%,平均回收率为100.6%。结论:研究结果表明,该法适用于常规批量测定血糖,且该法基本解决了溶血、脂血、黄疸对结果的影响。

关 键 词:双波长  双试剂  二点法  血糖
文章编号:1671-5098(2006)01-0032-02
收稿时间:2005-08-12

Method Study of Dual Wavelength Dual Reagents Two Points Method Detection Glucose
WANG Ke-jun, YAN Dong-hai, TAO Qian,et al. Method Study of Dual Wavelength Dual Reagents Two Points Method Detection Glucose[J]. Journal of Practical Medical Techniques, 2006, 13(1): 32-33
Authors:WANG Ke-jun   YAN Dong-hai   TAO Qian  et al
Abstract:Objective To establish a dual wavelength,dual reagents and two points method for detection of glucose.Methods Glucose was detected by the dual wavelength,dual reagents and two points method in BECKMANCOULTER LX20 automatic biochemical analyzer.Results The correlation coefficient is 0.998 6(r=0.998 6),compared to LX20 electrode method.The linear result is very good within the range of 22.2 mmol/L.The within-run CV is 2.11%~3.07%,the between-run CV is 3.57%.The average reclaimed rate is 100.6%.Conclusion The research result show that the method is much better in detecting routine glucose in batches and it is not influenced by the factor of hemlysis, lipemia, icterus etc.
Keywords:Dual reagent   Dual wavelength   Two point method   Glueose
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