Relationship between cell volume and cation content in thick ascending limb of rat kidney

We examined the relationship between the cell volume and cation concentration ([Na_i] and [K_i]) of isolated segments of rat medullary thick ascending limb (MAL) after incubation at 30°C in various isotonic solutions. When the tubules were incubated in a normal NaCl solution containing 5 mmol/l K⁺, addition of 1 mmol/l of ouabain increased [Na_i] and decreased [K_i] but did not change the total ([Na_i]+[K_i]) concentration (about 90 mEq/l) or tubular volume. After incubation in various K⁺-free solutions, the tubules were almost fully K⁺-depleted; their volume per unit of length was similar in the three solutions, although the choline Cl-treated tubules had a very low sodium content compared to the NaCl-and Na₂SO₄-treated tubules (8 vs. 97 and 95 mEq/l respectively). Ouabain altered neither volume nor [Na_i] of tubules incubated in choline Cl or Na₂SO₄ solution. Transfer of tubules from K⁺-free Na₂SO₄ or K⁺-free choline Cl solution into K⁺-free NaCl solution resulted in an increase in [Na_i] (by 29 and 97 mEq/l respectively) without much increase in tubular volume. A marked swelling of the tubules was only observed when the K⁺-free NaCl solution contained also ouabain. Under this condition, [Na_i] was comparable to the Na⁺ concentration of the incubation medium. After washing and incubation in a normal NaCl solution containing K⁺, the swollen tubules recovered their initial volume and restored Na⁺ and K⁺ concentration gradients across the cell membranes. The ([Na_i]+[K_i]) concentration centration measured in the tubules preincubated in choline Cl solution was always smaller than that of the tubules preincubated either in NaCl or Na₂SO₄ solutions, an observation suggesting that choline ions enter rat MAL cells. Barium (3 mmol/l) prevented tubular swelling. This inhibition corresponded to a smaller increase in [Na_i] than that observed in control tubules. Furosemide or bumetanide (even at 0.1 mmol/l) did not alter the increases in tubular volume and in Na⁺ content induced by ouabain. The data provide additional evidence that the isoosmotic swelling of MAL cells requires an almost full inhibition of Na⁺-pump activity and involves coupled net fluxes of Na⁺ and Cl^– ions.