| 封闭胸腺基质淋巴生成素受体对超敏原引起的气道炎症性应答的作用(英文) |
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| 引用本文： | 史丽云,周卸来,尹红萍,蔡玲斐,GARG,Hari,G,严杰. 封闭胸腺基质淋巴生成素受体对超敏原引起的气道炎症性应答的作用(英文)[J]. 杭州医学高等专科学校学报, 2009, 0(1): 7-13,F0002 |
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| 作者姓名： | 史丽云 周卸来 尹红萍 蔡玲斐 GARG Hari G 严杰 |
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| 作者单位： | [1]杭州师范大学基础医学部免疫学教研室 [2]Pulmonary and Critical Care Unit [3]Department of Medicine [4]Massachusetts General Hospital [5]Harvard Medical School [6]浙江大学医学院医学微生物学系 |
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| 基金项目： | 浙江省自然科学基金资助项目(Y204343);; 浙江省卫生科学计划项目(2004B162,2008QN026) |
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| 摘 要： | 目的为阐明胸腺基质淋巴生成素受体(Thymic stromal lymphopoietin receptor,TSLPR)在超敏原引起的气道炎症应答中的作用,并在小鼠模型中探讨局部封闭TSLPR用以缓解哮喘的可能性。方法对TSLPR抗体或同型抗体预处理,且以鸡卵白蛋白(OVA)诱导的各组小鼠,分别行气道浸润细胞的分类和记数、H&E和PAS的肺组织染色分析;并以酶联免疫吸附测定法(ELISA)检测支气管肺泡灌洗液中炎性细胞因子;进一步分析OVA激发的小鼠树突状细胞(DCs)的迁移能力和成熟状态。结果在小鼠OVA致敏前施以抗TSLPR抗体可显著减少气道粘液的分泌、嗜酸性粒细胞和淋巴细胞浸润;同时引发IL-4、IL-5水平的明显下降。而作为其机制之一,TSLPR的中和作用可阻止超敏原诱导的DCs的成熟和迁移。结论封闭TSLPR介导的信号通路可缓解哮喘小鼠的气道炎症反应,有望成为一项新的防治气道变应性疾病策略。
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| 关 键 词： | 胸腺基质淋巴生成素受体 哮喘 |
Effects of TSLPR blocking on allergen-induced airway inflammatory responses |
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| SHI Li-yun,;,; ZHOU Xie-lai,; YIN Hong-ping,; CAI Ling-fei,; GARG Hari G,; YAN Jie. Effects of TSLPR blocking on allergen-induced airway inflammatory responses[J]. Journal of Hangzhou Medical College, 2009, 0(1): 7-13,F0002 |
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| Authors: | SHI Li-yun, , ZHOU Xie-lai, YIN Hong-ping, CAI Ling-fei, GARG Hari G, YAN Jie |
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| Affiliation: | 1.Department of Immunology; Hangzhou Normal University; Hangzhou; Zhejiang 310036; China; 2.Pulmonary and Critical Care Unit; Department of Medicine; Massachusetts General Hospital; Harvard Medical School; Boston; MA 02114-2696; 3.Department of Medical microbiology; School of Medicine; Zhejiang University; Zhejiang 310058; China); |
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| Abstract: | Objective To define the role of Thymic stromal lymphopoietin receptor(TSLPR) in allergen-primed airway inflammatory responses,and to investigate the possibility of alleviating allergic disorders by locally inhibiting TSLPR in an asthmatic mice model. MethodsIn the anti-TSLPR antibody or isotype-immunoglobulin pretreated,ovalbumin(OVA)-primed mice,the airway infiltrated cells were fractioned and counted,and histologic analysis of lung tissues was conducted by staining with hematoxylin and eosin(H&E) and peri... |
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| Keywords: | thymic stromal lymphopoietin receptor asthma |
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