| 苦参碱对人黑素瘤细胞系增殖抑制的体外研究 |
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| 引用本文： | 姜孟臣,王火,陈虹. 苦参碱对人黑素瘤细胞系增殖抑制的体外研究[J]. 天津药学, 2003, 15(2): 9-11 |
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| 作者姓名： | 姜孟臣 王火 陈虹 |
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| 作者单位： | 中国人民武装警察部队医学院,天津,300162 |
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| 摘 要： | 目的：研究苦参碱(Matrine，Mat)对人黑素瘤细胞系生长的抑制效应，并探讨其作用机制。方法：用不同浓度的Mat加人体外培养人黑不经瘤(LiBr)细胞中，观察加药后细胞生长数量及其形态的变化；用MTT法检测Mat的细胞毒作用；用Hoechest33342和PI双荧光染色法检测细胞凋亡比率。结果：Mat明显抑制LiBr细胞生长；IC50值为0．8mg／ml；其抑制率与药物浓度呈剂量依赖关系；凋亡细胞的比例与药物浓度及作用时间呈正相关。结论：Mat能有效抑制LiBr细胞的增殖。
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| 关 键 词： | 苦参碱 人黑素瘤细胞 抑制效应 细胞生长数量 细胞增殖 细胞凋亡 |
| 文章编号： | 1006-5687(2003)02-0009-03 |
Effect of Matrine on proliferation of Human LiBr cellline in vitro |
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| Jiang Mengchen,Wang Huo,Chen Hong. Effect of Matrine on proliferation of Human LiBr cellline in vitro[J]. Tianjin Pharmacy, 2003, 15(2): 9-11 |
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| Authors: | Jiang Mengchen Wang Huo Chen Hong |
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| Abstract: | Objective: To study the effects and mechanism of Matrine on LiBr cell line. Methods: Matrine of different concentrations were added into the medium in which the cells were cultured. The changes of cell morphology were observed by phase contrast microscope. Cellulotoxic activity of Matrine was detected by MTT. The number of apoptotic cells was quantitatively assessed by fluorescent light microscope. Results: The growth of LiBr cell line was remarkably inhibited by Matrine. The IC 50 values of Matrine for LiBr cells was 0.8 mg/ml, The inhibited and the rate of apoptotic cells were related with the drug concentration. Conclusion: Matrine can effectively inhibit the multiplication of LiBr cell line. |
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| Keywords: | Matrine LiBr cell line Cell proliferation |
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