严重烧伤后小鼠腹腔巨噬细胞（Mφ）CD14表达的变化

目的 探讨烧伤后早期腹腔Mφ CD14分子的动态变化。方法 采用小鼠20％TB-SAⅢ度烧伤模型，分离和培养小鼠腹腔Mφ，用免疫组织化学、RT—PCR和ELISA方法，观察了烧伤复苏组和延迟复苏组伤后24h内不同时间腹腔Mφ表达CD14和CD14 mRNA、分泌肿瘤坏死因子(TNF)和白介素-6(IL-6)的变化。结果 烧伤复苏组和延迟复苏组在伤后1h腹腔Mφ表达CD14和分泌TNFα、IL-6明显增加，这种改变在伤后24h内持续。结论烧伤能增加Mφ CD14 mRNA的表达，从而使Mφ易于被很低浓度内毒素(LPS)激活，Mφ的致敏和激活使其在伤后过度炎症反应的发生发展中起重要作用。

Increased expression of peritoneal macrophage CD14 in severely burned mice

OBJECTIVE: To observe the dynamic changes in peritoneal macrophage (Mphi) CD14 expression in severely burned mice. METHODS: The mice, being inflicted with 20% TBSA III degree burn, were randomly divided into two groups: Group A: The mice in this group received fluid resuscitation instantaneously after burn. Group B: The mice in this group received delayed fluid resuscitation at 6hr postburn. The Mphis at different postburn time points were obtained in two groups for the detection of their expression of CD14 and CD14 mRNA with immunohistochemical and RT -- PCR analysis, and also for the determination with ELISA of the changes in TNF and IL -- 6 levels in the supernatant of their culture. RESULTS: The expression of CD14 and the levels of TNF and IL -- 6 increased obviously in both groups from 1 to 24 hrs postburn. CONCLUSION: Burn injury would induce the increase of the expression of Mphi CD14 mRNA in mice. As a result, the Mphi became more readily to be activated by low levels of LPS. The activated Mphi might play an important role in the development of postburn SIRS.