COX-2抑制剂对膀胱肿瘤的抑制作用

目的探讨环氧化酶（COX-2）抑制剂塞来昔布对小鼠膀胱肿瘤的抑制作用及其机制。方法利用BTT739膀胱癌细胞株和T739小鼠建立膀胱肿瘤动物模型。小鼠随机分为实验组和对照组，每组10只。造模后第2天起实验组塞来昔布混悬液灌胃（20mg／kg），对照组生理盐水灌胃。观察肿瘤的生长情况并绘制肿瘤生长曲线。4周后，处死小鼠，切取肿瘤，称量瘤重，计算抑瘤率。肿瘤行常规病理学检查，观察肿瘤细胞的形态学变化，用免疫组化法观察肿瘤细胞中的COX一2、血管内皮生长因子（VEGF）的表达情况。结果对照组平均瘤重为（3．27±1．89）g，实验组为（1．10±0．52）g，两组差别有统计学意义（P〈0．05）；抑瘤率为66，4％。实验组和对照组肿瘤潜伏期（7．10±2．28）dvs（7．20±2．71）d]差别无显著性（P〉0．05）。肿瘤生长曲线上可看出两组肿瘤生长自第2周开始显示差异，直至实验结束。显微镜下实验组标本见大片凝固性坏死区，而对照组标本仅可见局灶性坏死。免疫组化见COX-2、VEGF是表达于肿瘤细胞的细胞浆和／或细胞膜。实验组与对照组COX-2、VEGF的表达水平有统计学差异（P〈0．05或P〈0．01）。且COX-2与VEGF的表达呈正相关。结论塞来昔布对小鼠膀胱肿瘤有抑制作用，其机制可能为通过抑制COX-2来抑制血管形成，从而抑制肿瘤生长。

The effect of COX-2 inhibitor on bladder cancer

Objective To study the inhibiting effects of celecoxib on mice bladder cancer and investigate the mechanism. Methods Models were established with T739 mice and BTT739 cell line. Mice were randomly divided into the control group （CG） and the test group （TG）, with 10 in each one. From the second day after operation, celecoxib in suspension at 20 mg/kg was administered to mice in TG orally, while saline to the mice in the CG. During the test, the growth of tumor mass was observed and the growth curve was drawn. 4 weeks later, the mice were to resect tumors, compare their weight and conduct routine pathology examination. The expression of COX-2 and VEGF was observed with immunohistochemistry. Results The mean weight of test group was （1.10±0.52）g, control group （3.27±1.89）g, which was significant in statistics （P〈0.05）. The difference of latent period between TG and CG was not significant statistically （7.10±2.28）d vs. （7.20±2.71）d] （P〉 0.05）. The growth curve showed that there was difference in tumor volume between CG and TG, and it lasted to the end of the test. Pathologically, large necrosis area could be seen in TG, while not in CG. In immunohistochemistry, COX-2 and VEGF were positive in cell plasma and/or cell membrane. The expression of COX-2 and VEGF in TG was statistically different from that in CG （P〈0.05 or P〈0.01）. And there was a positive correlation between the expression of COX-2 and VEGF. Conclusion Celecoxib inhibits COX-2 to constrain angiogenesis so as to restrain bladder cancer.