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| 8-Br-cAMP和槲皮素对Eca-109细胞iNOS基因拷贝及Caspase-3表达的影响 | |
| 引用本文： | 任伟宏,宫璀璀,吴景兰,赵志娟,顾侦芳,苏安英.8-Br-cAMP和槲皮素对Eca-109细胞iNOS基因拷贝及Caspase-3表达的影响[J].郑州大学学报(医学版),2003,38(5):684-686. |
| 作者姓名： | 任伟宏 宫璀璀 吴景兰 赵志娟 顾侦芳 苏安英 |
| 作者单位： | 1. 河南中医学院第一附属医院检验科,郑州,450000 2. 解放军第153医院检验中心,郑州,450042 3. 郑州大学分子细胞生物学研究中心,郑州,450052 4. 河北工程学院医学部,邯郸,056029 |
| 基金项目： | 河南省自然科学基金资助项目 9840 2 180 0，河南省教育科研基金资助项目2 0 0 0 180 0 7 |
| 摘 要： | 目的 :观察 8 Br cAMP和槲皮素对Eca 10 9细胞iNOS基因拷贝及Caspase 3表达的影响。方法 :将贴壁培养的Eca 10 9细胞随机分成 3组 ,Br组 :加 8 Br cAMP至终浓度为 2× 10 5mol/L ;Q组 :加槲皮素至终浓度为 4 3μmol/L ;C组 :不加任何药物 ,只加入新培养液培养。 3组细胞同时培养 4 8h。采用原位斑点印迹法显示iNOS基因拷贝和mRNA的表达 ,采用细胞免疫化学技术显示Caspase 3的表达。结果 :Br和Q组较C组iNOS基因拷贝和mRNA表达信号较强。Br和Q组细胞内Caspase 3免疫反应 (IR)性较强 ,Br和Q组与对照组相比 ,存在显著性差异 (P 0 .0 1) ;Br组的Caspase 3 IR强于槲皮素组 (P <0 .0 1)。结论 :8 Br cAMP和槲皮素皆可上调iNOS基因的扩增和表达 ,最终可都通过上调Caspase 3的表达 ,诱导Eca 10 9细胞凋亡。 |
| 关 键 词： | 细胞凋亡 iNOS Caspase-3 Eca-109细胞 |
Expression of iNOS gene and Caspase-3 in Eca-109 cell apoptosis induced by 8-Br-cAMP and quercetin |
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| REN Weihong ,GONG Cuicui ,WU Jinglan ,ZHAO Zhijuan ,GU Zhenfan ,SU Anying.Expression of iNOS gene and Caspase-3 in Eca-109 cell apoptosis induced by 8-Br-cAMP and quercetin[J].Journal of Zhengzhou University: Med Sci,2003,38(5):684-686. | |
| Authors: | REN Weihong GONG Cuicui WU Jinglan ZHAO Zhijuan GU Zhenfan SU Anying |
| Institution: | REN Weihong 1),GONG Cuicui 2),WU Jinglan 3),ZHAO Zhijuan 1),GU Zhenfan 1),SU Anying 4) 1)Department of Clinical Laboratory,the First Affiliated Hospital,Henan Traditional Chinese Medicine College,Zhengzhou 450000 2)Laboratory Medicine Center,the PLA No.153 Hospital,Zhengzhou 450042 3)Molecular Cell Biology Research Center,Zhengzhou University,Zhengzhou 450052 4)Medical Center,Hebei Engineering College,Handan 056029 |
| Abstract: | Aim:To study the amplification and expression of iNOS gene and its role in Eca 109 cell apoptosis.Methods: The cultured Eca 109 cells were randomly divided into 3 groups: 8 Br cAMP (Br)group, Quercetin (Q)group, and Control (C)group with no any drugs.The 3 group cells were cultured for 48 h under the same condition.The expression and copies of iNOS gene were demonstrated by DNA/RNA dot blot and the caspase 3 immunoractivity(IR) by immunohistochemistry.Results: Compared with the C group, the gene copies and mRNA dot blot signals and caspase 3 IR were stron ger in the Br and Q groups ( P <0.01); Caspase 3 IR was higher in Br group than that in Q group( P <0.01).Conclusion: Both 8 Br cAMP and quercetin could up regulate iNOS gene copies and expression, and finally induce Eca 109 cell apoptosis through up regulating expression of Caspase 3. |
| Keywords: | apoptosis iNOS Caspase 3 Eca 109 cell line |
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