| Caspase-3在UVB诱导人皮肤成纤维细胞凋亡中的作用 |
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| 引用本文： | 徐浩翔,闫言,李莉,王宝玺.Caspase-3在UVB诱导人皮肤成纤维细胞凋亡中的作用[J].中国麻风皮肤病杂志,2012(10):697-700. |
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| 作者姓名： | 徐浩翔 闫言 李莉 王宝玺 |
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| 作者单位： | [1]中国医学科学院北京协和医学院皮肤病研究所,南京210042 [2]中国医学科学院北京协和医学院北京协和医院皮肤科,北京100730 [3]首都医科大学附属北京朝阳医院皮肤科,北京100043 |
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| 基金项目： | 国家自然科学基金（编号：81101207）教育部高校博士点基金（编号：20111106120052） |
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| 摘 要： | 目的：评价caspase-3在UVB诱导皮肤成纤维细胞凋亡中的作用。方法：皮肤成纤维细胞经150mJ／cm2 UVB照射后，用MTF法检测细胞活性，用Hoechst33258染色法检测细胞凋亡，用抑制剂Z-DEVD-FMK抑制caspase-3活性后，检测凋亡细胞数量。结果：UVB明显抑制成纤维细胞的活性，并导致细胞凋亡，并呈时间-效应关系；加入抑制剂Z-DEVD-FMK抑制了UVB导致的细胞凋亡。结论：Caspase-3在UVB照射诱导皮肤成纤维细胞凋亡中发挥重要作用。
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| 关 键 词： | 紫外线B 人皮肤成纤维细胞 caspase-3 |
The role of caspase- 3 in human dermal fibroblasts apoptosis induced by UVB irradiation |
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| Institution: | XU Hao-xiang, YAN Yan, LI Li, et al. Institute of Dermatology, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Nanjing, 210042 |
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| Abstract: | Objective： To evaluate the role of caspase - 3 in skin fibroblasts apoptosis induced by UVB irradi- ation. Methods： After the human skin fibroblasts were irradiated by UVB, the cell viability measured by MTY assay and the apoptosis of the cells tested with Hoechst staining method. The number of cell apoptosis was counted after the activity inhibited by adding an inhibitor Z- DEVD - FMK. Results： UVB irradiation markedly inhibited the fibro- blasts viability and resulted in apoptosis, which was in a time - effect manner. The apoptosis induce by UVB irradi- ation was inhibited by adding Z- DEVD - FMK. Conclusion： Caspase - 3 plays an important role in apoptosis of human dermal fibroblasts induced by UVB irradiation. |
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| Keywords: | ultraviolet B human skin fibroblasts caspase - 3 |
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