Influence of alpha-naphthylisothiocyanate (ANIT) on microsomal cytochrome P-450, protein and phospholipid content in rat liver

In rats suffering from ANIT induced cholestasis plasma and microsomal phospholipid content was measured using lipid extraction, thin-layer chromatography and phosphorus determination. Microsomal protein and cytochrome P-450 content were also measured. Plasma phospholipids were found to be increased without similar changes within the liver. In ANIT-treated animals the hepatic microsomal protein content was increased whereas the microsomal phospholipid and eytochrome P-450 content remained unchanged.