青年献血人群HBV/OBI感染者血清中IL-10表达水平的分析

目的检测乙型肝炎病毒感染者及隐匿性乙型肝炎病毒感染(OBI)者外周血中IL-10的表达水平。方法收集2013年6月~2015年6月期间陕西省血液中心采集的年龄范围在20~40岁的无偿献血者血液标本，选择HBsAg阳性标本作为HBV组；选择HBsAg阴性/HBV DNA阳性，同时经随访排除窗口期的血液标本作为OBI组，采用酶联免疫法(ELISA)检测48例OBI感染者，37例HBV感染者血清中IL-10的水平，并分析其与丙氨酸氨基转移酶(ALT)、乙肝病毒血清学标志物之间的相关性。结果OBI组血清IL-10含量为21.9±0.7 pg/ml，显著低于HBV组40.4±10.4 pg/ml，差异有统计学意义(F=4.062，P=0.047)；OBI组、HBV组血清中ALT含量分别为18.3 ±1.3 U/L和18.4±1.5 U/L，组间差异无统计学意义(F=1.131，P=0.992)。根据ALT水平的高低，进一步将HBV组和OBI组献血者分为ALT≤20 U/L和20 U/L

Analysis of IL-10 in Serum of Blood Donors with OBI/HBV Infection among Young Adults

ObjectiveTo compare the level of cytokines IL-10 and its function in serum of OBI/HBV infected donors in Xi’an．MethodsSelected serum samples from donors aged 20~40 years old in Shaanxi Blood Center from June 2013 to June 2015．Samples with HBsAg positive were divided into HBV group，while samples with HBsAg negative/ HBV DNA positive，excluded from window period，were divided into OBI group．The levels of IL-10 were detected from the serum of 37 HBV donors and 48 OBI donors by ELISA．Also analyzed the correlation between levels of IL-10 and ALT or the five HBV serological markers．ResultsCompared with the HBV donors (40.4±10.4)pg/ml，the levels of IL-10 of OBI donors (21.9±0.7)pg/ml were significantly decreased (F=4.062，P=0.047)．The level of ALT had no significant difference between OBI group (18.3 ±1.3)U/L and HBV group (18.4±1.5)U/L．The express level of IL-10 had no significant difference between ALT≤20 U/L and 20 U/L