儿童肺炎支原体感染IgM抗体和DNA检测结果的应用分析

目的 通过对肺炎支原体IgM抗体和DNA检验结果分布规律的分析,探讨其对儿童肺炎支原体感染诊断的应用。方法 回顾性分析2013～2014年全年中国医科大学附属盛京医院0～16岁儿童肺炎支原体IgM抗体(MP-IgM)和肺炎支原体DNA(MP-DNA)检测数据。其中MP-IgM 114 374人次; MP-DNA 8 014人次,分析性别、年龄、季节等与该指标阳性率的关系; 收集同时进行MP-IgM和MP-DNA检测的患者5106例,分析不同年龄两指标阳性结果的一致性。结果 ①不同性别、年龄和季节MP-IgM和MP-DNA检测阳性率不同,女性的检测阳性率均高于男性; 1～3岁的MP-IgM和7～12岁的MP-DNA阳性率分别高于其他年龄段; MP-IgM检测阳性率以春秋季为高,而MP-DNA则以夏秋季为高,差异均有统计学意义(P＜0.001)。②不同标本类型MP-DNA阳性率有所差异,以肺泡灌洗液的阳性检出率最高,为49.95%。③MP-IgM和MP-DNA阳性一致性与年龄有关,以4～6岁和7～12岁符合率较高,分别为35.2%和45.8%。结论 ①儿童肺炎支原体感染发病人群以学龄前和学龄期儿童为主,女性多于男性,秋季高发。②肺泡灌洗液是MP-DNA检测最佳标本类型。③＜1岁和1～3岁宜采用MP-DNA和MP-IgM联合检测提高诊断率。

Application Analysis of IgM Antibody and DNA Test Results in Children Mycoplasma Pneumonia Infection

Objective To analyze the results of distribution of mycoplasma pneumoniae IgM antibody and DNA test,and explore its application in diagnosis of Mycoplasma pneumoniae infection in children.Methods Retrospectively analysed mycoplasma pneumoniae antibody IgM(MP-IgM)and mycoplasma pneumoniae DNA(MP-DNA)results,114 374 passengers and8 014 passengers,in 0～16 year-old children of Shengjing Hospital Affiliated toChina Medical University from year 2013 to 2014.Analyzed of the relationship between gender,age,season and the positive rate of the MP-IgM and MP-DNA.Collected 5 106 patients with MP-IgM and MP-DNA results simultaneously and analyze the consistency between two positive indexes in different ages.Results ①MP-IgM and MP-DNA positive rate varied in sex,age and season,the positive rate in female was higher than male.The positive rates of MP-IgM and MP-DNA at the age of 1～3 and 7～12 years old were higher than other agegroups.The positive rate of MP-IgM in spring and autumn was higher,while MP-DNA was higher in summer and autumn and all differences were statistically significant(P＜ 0.001).②The positive rate of MP-DNA differed in specimens,the bronchoalveolar lavage fluid had the highest MP-DNA positive rate,49.95%.③The positive consistency of MP-IgM and MP-DNA was associated with age,with 4～6 and 7～12 year-olds the highest,respectively 35.2% and 45.8%.Conclusion ①Incidence of children pneumonia mycoplasma infectionwas mainly in preschool and school-aged children,more female than male,and autumn highest.②The detection of MP-DNA in bronchoalveolar lavage fluid was thebest sample type.③For ＜1 and 1～3 year-olds,combined detection of MP-DNA and MP-IgM can improve diagnostic rates.