儿童特发性血小板减少性紫癜Th亚群细胞因子的测定及意义

目的研究外周血Th亚群细胞因子IFN-γ，IL-4和TGF-β1在急、慢性儿童特发性血小板减少性紫癜中的浓度及临床意义。方法应用流式细胞术检测外周血IFN-γ和IL-4表达水平，采用酶联免疫(ELISE)法检测外周血单个核细胞生成的TGF-β1水平，并与24例正常对照组检测进行比较。结果aITP患儿Th1细胞因子IFN-γ阳性百分比高于正常对照组(P<0.05)，cITP患儿Th1细胞因子IFN-γ阳性百分比较对照组显著降低(P<0.01)。aITP患儿Th2细胞因子IL-4与正常对照组比较差异无统计学意义(P>0.05)，cITP患儿Th2细胞比对照组显著升高(P<0.05)。aITP和cITP Th3细胞因子TGF-β1水平均较正常对照组明显降低，cITP较aITP显著降低，差异有统计学意义(P<0.05)。结论儿童急、慢性ITP中Th细胞因子对其发病机制研究及鉴别诊断有重要价值。

Determination and Clinical Significance of Th Subsets Cytokines in ITP of Children

ObjectiveTo study the determination and clinical significance of Th cytokines subsets of IFN-γ，IL-4 factor and TGF-β1 in acute and chronic idiopathic thrombocytopenic purpura．MethodsThe level of IFN-γ，IL-4 and TGF-β1 (produced by peripheral blood mononuclear cells)were detected by flow cytometry and enzyme-linked immunosorbent assay (ELISE)，respectively，in patients and 24 controls．ResultsThe positive percentage of factor IFN-γ in children with aITP was higher than that in the controls (P<0.05)．The positive percentage of factor IFN-γ in children with cITP was significantly lower than the controls (P<0.01)．The level of IL-4 had no statistical significance in children with aITP compared with controls．The level of IL-4 was higher significantly in children with cITP than that in controls (P<0.05)．The level of TGF-β1 in children with aITP and cITP was lower significantly than that in controls，that was more significance in cITP than that in aITP (P<0.05)．ConclusionThe state of Th cytokines in children with acute and chronic ITP is of great value in the understanding of the pathogenesis and differential diagnosis．