Multiplex PCR assay for rapid detection and genotyping of Helicobacter pylori directly from biopsy specimens |
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| Authors: | Chattopadhyay Santanu Patra Rajashree Ramamurthy T Chowdhury Abhijit Santra Amal Dhali G K Bhattacharya S K Berg Douglas E Nair G Balakrish Mukhopadhyay Asish K |
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| Affiliation: | National Institute of Cholera and Enteric Diseases, Calcutta 700010, India. |
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| Abstract: | We developed and evaluated a simple, novel multiplex PCR assay for rapid detection of Helicobacter pylori infection and for the determination of vacA and cagA genotypes directly from gastric biopsy specimens. This assay did not require culturing of strains or extraction of DNA from biopsy samples. This multiplex PCR assay would be of particularly great value for laboratories in developing countries. |
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