肾小球肾炎患者WBC流变性和CD18表达及血清sICAM—1浓度测定及其意义

探讨ＷＢＣ流变性和细胞粘附分子（ＣＡＭｓ）与肾小球肾炎病情变化的关系。方法采用红细胞变形能力测定仪、体外。栓血小板粘附两用仪及酶联免疫吸附法（ＥＬＩＳＡ），分别检测了４０例急性肾小球肾炎患者、４２例慢性肾小球肾炎患者和４５例健康人外周血白细胞滤过指数（ＬＥＩ）、白细胞粘附率（ＬＡＲ）、白细胞ＣＤ１８表达及血清可溶性细胞间粘附分子一１（ＳＩＣＡＭ－１）浓度的变化。结果肾小球肾炎患者ＬＦＩ，ＬＡＲｔ，白细胞ＣＤ１８表达和ｓＩＣＡＭ－１浓度均明显增高，与对照组比较差异有极显著性（Ｐ均＜０．００１），且慢性肾小球肾炎患者各指标增高较急性肾小球肾炎患者更明显（Ｐ均＜０．００１）。结论白细胞流变性异常及ＣＤ１８表达、血清ｓＩＣＡＭ－１浓度增高与肾小球肾炎病情变化有密切关系。

Significance of the leukocyte rheologic characteristics and expression of leukocyte CD_(18) and detection of sICAM-1concentration in patients with glomerulonephritis

Objective: To explore the correlation between the leukocyte rheologic characteristics, cell adhesion molecules andglomerulonephritis .Methods The leukocyte CD_18 expression, serum soluble cell intercellular adhesion molecule - 1 (sICAM - 1 )concentration, leukocyte filtration index(LFI)and leukocyte anhesion rate (LAR) were measued in 40 acute glomerulonephritis,42 chronic glomerulonephritis patients and 45 hpalthy controls by the erythrocyte deformability apparatus, thrombus andpletelet adhesion dual-Purpose apparatus and enzyme-linked immunosorbent assay (ELISA).Results teukocyte CD_18 expression, sICAM-l concentration, LFI and LAR in patients were significantly higher than those in controls(P<0.001),and the increase of all indicators in chronic glomerulonephritis patients was more obvious than that in acute patients (P<0.001).Con-clusion The increase of the leukocyte CD_18 expression, sICAM-1 concentration and the abnormalities of leukocyte rheological characteristics were closely related to the glomerulonephritis patients condition.