| 兔退变椎间盘中BNIP3的免疫组织化学研究 |
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| 引用本文： | 刘杰,彭娜,王建.兔退变椎间盘中BNIP3的免疫组织化学研究[J].中国局解手术学杂志,2012(1):3-5. |
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| 作者姓名： | 刘杰 彭娜 王建 |
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| 作者单位： | [1]解放军93197部队,辽宁沈阳110044 [2]第三军医大学新桥医院骨科,重庆400037 [3]第三军医大学新桥医院手术室,重庆400037 |
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| 基金项目： | 国家自然科学基金项目（30872601） |
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| 摘 要： | 目的探讨兔退变椎间盘中BNIP3蛋白的表达情况。方法建立兔椎间盘穿刺退变模型,分别培养2、4、8周后对目的椎间盘进行组织HE染色、番红O染色及BNIP3免疫组织化学染色,与正常椎间盘随机对照,检测椎间盘退变程度及BNIP3蛋白表达情况。结果成功建立兔椎间盘退变模型,随椎间盘退变程度加重,BNIP3蛋白在中央髓核组织中的阳性表达逐渐增强。结论兔椎间盘退变过程中,BNIP3蛋白表达增强诱导髓核细胞死亡增加。
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| 关 键 词： | 椎间盘 退变 BNIP3 免疫组织化学 |
Immunohistochemistry research on BNIP3 expressions of degenerated intervertebral disc in rabbit |
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| LIU Jie,PENG Na,WANG Jian.Immunohistochemistry research on BNIP3 expressions of degenerated intervertebral disc in rabbit[J].Journal of Regional Anatomy and Operative Surgery,2012(1):3-5. |
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| Authors: | LIU Jie PENG Na WANG Jian |
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| Institution: | 197 Unit of PLA,Shengyang Liaoning 110044,China) |
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| Abstract: | Objective To explore the expressions of BNIP3 protein of degenerated intervertebral disc in rabbit.Methods The disc degenerative model of rabbit was established.The disc was isolated after raising for 2,4,8 weeks and HE stain,safranin O stain and BNIP3 immunohistochemical stain were performed on targeted disc respectively.Comparision was made with normal disc to determine the degenerative degree and BNIP3 protein expressions.Results The disc degenerative model of rabbit was successfully established.It was demonstrated that the positive expressions of the BNIP3 in nucleus pulposus were increased dramatically as the degeneration of the disc becoming more severe.Conclusion The increased expressions of BNIP3 in disc degeneration will induce necrocytosis in nucleus pulposus. |
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| Keywords: | intervertebral disc degeneration BNIP3 immunohistochemistry |
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