| 柔红霉素产生菌Streptomyces coeruleorubidus SIPI-1482中dnmV因的克隆及阻断 |
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| 引用本文： | 尚珂 胡又佳 朱春宝 朱宝泉 谢丽萍. 柔红霉素产生菌Streptomyces coeruleorubidus SIPI-1482中dnmV因的克隆及阻断[J]. 中国医药工业杂志, 2006, 37(5): 307-310 |
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| 作者姓名： | 尚珂 胡又佳 朱春宝 朱宝泉 谢丽萍 |
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| 作者单位： | 上海医药工业研究院,上海200040 |
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| 摘 要： | 从柔红霉素产生菌SIPI-1482菌株基因组DNA中PCR扩增得dnmV及其上游dnmU基因片段。利用同源重组对SIPI-1482菌株的dnmV基因进行基因阻断，得到一株遗传稳定的破坏于。验证了在该菌株中进行同源重组所需交换臂长度。
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| 关 键 词： | 柔红霉素 同源重组 基因阻断 |
| 文章编号： | 1001-8255（2006）05-0307-04 |
| 收稿时间： | 2005-09-07 |
| 修稿时间： | 2005-09-07 |
Cloning and Disruption of dnm V from Daunorubicin-producing Strain Streptomyces coeruleorubidus SIPI-1482 |
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| SHANG Ke, XIE Li-Ping, HU You-Jia, ZHU Chun-Bao, ZHU Bao-Quan. Cloning and Disruption of dnm V from Daunorubicin-producing Strain Streptomyces coeruleorubidus SIPI-1482[J]. , 2006, 37(5): 307-310 |
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| Authors: | SHANG Ke XIE Li-Ping HU You-Jia ZHU Chun-Bao ZHU Bao-Quan |
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| Affiliation: | Shanghai Institute of Pharmaceutical Industry, Shanghai 200040 |
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| Abstract: | dnmV was PCR amplified along with its upstream dnmU gene from genomic DNA of Streptomyces coeruleorubidus SIPI-1482, which was one of the daunorubicin-producers. Homologous recombination was used for dnmV gene disruption in SIPI-1482, and one genetically stable disruptant was obtained. The homologous DNA fragment which was sufficient in length for recombination to occur in this strain was identified. |
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| Keywords: | daunorubicin homologous recombination gene disruption |
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