虾仁制品中金黄色葡萄球菌及其肠毒素的检测研究

目的分析研究虾仁制品中金黄色葡萄球菌及其肠毒素污染状况。方法金黄色葡萄球菌按GB/T4789.10-2003检测,金黄色葡萄球菌肠毒素采用mini-VIDAS全自动荧光酶标免疫分析仪进行测定。结果速冻虾仁中金黄色葡萄球菌的检出率为68.2%,从检出的金黄色葡萄球菌中产肠毒素菌株的检出率为40.0%;脱水虾仁中金黄色葡萄球菌的检出率为31.8%,从检出的金黄色葡萄球菌中产肠毒素菌株的检出率为100.0%。结论虾仁制品金黄色葡萄球菌及其肠毒素的污染严重,应加强对虾仁制品中金黄色葡萄球菌肠毒素的检测。

The detection of staphylococcus aureus and enterotoxin in byproducts of shrimps

Objective The study was made to investigate and analyze the status of the contamination of staphylococcus aureus and enterotoxin in bioproducts of shrimp.Methods Staphylococcus aureus was tested according to GB/T4789.10-2003 and its enterotoxin was determined by mini-VIDAS Automatic Luciferaseimmunization Analyzer.Results It is found out that detection rate of staphylococcus aureus is 68.2%for quick freezing shrimps,with detection rate of 40.0%for enterotoxin Staphylococcus aureus thereof.Detection rate of staphylococcus aureus is 31.8%for dehydration shrimps,with detection rate of 100.0%for enterotoxin Staphylococcus aureus thereof.Conclusion It revealed that contamination of staphylococcus aureus and enterotoxin in the shrimp byproducts is serious and enterotoxin detection of Staphylococcus aureus in the shrimps byproducts should be strengthen.