| 巴西蘑菇Agaricus blazei Murill水溶性多糖的分离纯化 |
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| 引用本文： | 林跃鑫,叶竹秋,黄谚谚,谢华玲.巴西蘑菇Agaricus blazei Murill水溶性多糖的分离纯化[J].药物生物技术,2003,10(4):238-241. |
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| 作者姓名： | 林跃鑫 叶竹秋 黄谚谚 谢华玲 |
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| 作者单位： | 福建师范大学生物工程学院,福建,福州,350007 |
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| 基金项目： | 福建省教育厅资助项目,项目编号 :JB980 13 |
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| 摘 要： | 从巴西蘑菇子实体中提取的水溶性粗多糖经过脱蛋白、脱色等步骤获得半纯品多糖。依次采用DE—AB纤维素和Sepharose CL-4B柱层析进一步纯化后，获得5种水溶性多糖组分。其中主要组分AB-I、AB-Ⅱ-b和AB-Ⅲ-b得率分别为3．3％、1．52％、0．85％。Sephadex G-l00凝胶层析、旋光度和HPLC鉴定各组分已达到层析纯。
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| 关 键 词： | 巴西蘑菇 多糖 提取 纯化 Agaricus-blazei-Murill水溶性多糖 |
| 文章编号： | 1005-8915(2003)04-0238-04 |
Extraction and Purification of Water-Soluble Polysaccharides from Agaricus blazei Murill Fruit Body |
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| LIN Yue-xin,YE Zhu-qiu,HUANG Yan-yan,XIE Hua-ling.Extraction and Purification of Water-Soluble Polysaccharides from Agaricus blazei Murill Fruit Body[J].Pharmaceutical Biotechnology,2003,10(4):238-241. |
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| Authors: | LIN Yue-xin YE Zhu-qiu HUANG Yan-yan XIE Hua-ling |
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| Abstract: | Crude polysaccharide was obtained from Agaricus blazei Murill fruiting body by hot water extraction, ethanol precipitation and pronase treatment. Five fractions were identified from the crude polysaccharide by DEAE-cellulose chromatography and Sepharose CL-4B gel filtration. Three main fractions (AB-I, AB-II-b, AB-III-b) were collected finally. The total extraction rates of AB-I, AB-II-b, and AB-III-b were 3.3%, 1.52% and 0.85% respectively. Sephadex G-100 gel filtration,optical rotation examination and HPLC detection showed that the polysaccharides were purified. |
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| Keywords: | Agaricus blazei Murill Polysaccharide Extraction Purification |
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