水蛭和丹参对血管成形术后平滑肌细胞增殖影响的实验研究

目的：探讨腔内血管成形术（ＴＡ）后再狭窄的预防方法。方法：将６０支髂动脉（３０只家兔）随机分为３组（每组２０支髂动脉）：水蛭组、丹参组和对照组，造成实验性动脉粥样硬化狭窄并进行ＴＡ后，水蛭组每天饲喂２ｇ水蛭粉和普通饲料，丹参组每天饲喂２ｇ丹参粉和普通饲料，对照组每天只饲喂普通饲料，３０天后，进行血管造影和病理形态学检查。结果：（１）水蛭组髂动脉内膜厚度（４０．１±９．８μｍ）显著小于丹参组（４８．

Experimental Study of Hirudo and Salvia miltiorrhiza on Smooth Muscle Cell Hyperplasia after Balloon Angioplasty

OBJECTIVE: To find the preventive method of restenosis after transluminal angioplasty (TA). METHODS: Sixty rabbit iliac arteries of 30 rabbits were divided randomly into three groups, group A, B and C, 20 in each. After experimental atherosclerotic stenosis and TA performing, ordinary forage plus 2 g of hirudo powder was fed to the group A every day, to the group B, 2 g Salvia miltiorrhiza powder was added and to the group C, ordinary forage only. Angiography and pathomorphological examination were carried out 30 days later. RESULTS: (1) Hirudo significantly reduced the thickness of iliac intima as compared with the other two groups (P < 0.05), the thickness in the group A, B and C was 40.1 +/- 9.8 microns, 48.2 +/- 8.2 microns and 69.3 +/- 9.2 microns respectively. (2) The incidence of restenosis after TA in both group A (31.4%) and group B (48.4%) were significantly lower than that in the group C (62.8%, P < 0.05). And significant difference was also found in comparison of group A and group B (P < 0.05). (3) Ultrastructure change examined by transmission electron microscopy showed that less rough endoplasmic reticulum and more myofilaments in well differentiated smooth muscle cell (SMC) of the arterial intima in the group A and B, while in the group C, more rough endoplasmic reticulum, more synthetic cells were seen in SMC. CONCLUSIONS: Hirudo and red sage root could effectively inhibit the hyperplasia of SMC in arterial intima and reduce the intima thickness and the incidence of restenosis after TA significantly, thus might play a role in the prevention of restenosis after TA. The effect of hirudo is more potent than that of red sage root.