白藜芦醇对脑缺血再灌注损伤的抗氧化活性及线粒体保护作用的研究

目的:研究白藜芦醇(Resveratrol)对局灶性脑缺血再灌注损伤大鼠的抗氧化及线粒体保护作用。方法:采用栓线法复制局灶性脑缺血(MCAO)再灌注损伤模型,观察白藜芦醇对大鼠脑组织匀浆中超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)和乳酸(LD)、一氧化氮(NO)的影响;检测脑组织线粒体组分中的MDA、GSH含量及SOD、ATP酶活性的变化。结果:白藜芦醇对大鼠MCAO 2 h再灌注24 h后脑组织及其线粒体组分中SOD活性和GSH含量的降低有明显的抑制作用,能抑制大鼠脑组织和脑组织线粒体组分中MDA的增加,能抑制大鼠脑组织LD、NO含量的增加,并可明显提高脑组织线粒体组分中Na -K -ATPase、Ca2 -Mg2 -ATPase活性。结论:白藜芦醇对局灶性脑缺血再灌注损伤大鼠的保护作用的机制可能与其抗脂质过氧化和保护线粒体的作用有关。

Anti-oxidative and mitochondria protective effects of resveratrol on focal cerebral ischemia-reperfusion injury in rats

AIM: To investigate anti-oxidative and mitochondria protective effects of resveratrol on focal cerebral ischemia-reperfusion injury in rats. METHODS: Middle cerebral artery occlusion(MACO) was used to induce focal cerebral ischemia-reperfusion model.After 24 h reperfusion,MDA,GSH,NO,LD content and SOD activity in brain homogenate were determined,MDA,GSH content and SOD,ATPase activity in mitochondria were also determined. RESULTS: Resveratrol significantly inhibited the increase in MDA,NO,LD content and the decrease in SOD activity and GSH content in brain after cerbral ischemia-reperfusion.Resveratrol also significantly inhibited the increase in MDA and the decrease in activity of SOD,Na~ -K~ -ATPase,Ca~(2 )-Mg~(2 )-ATPase in mitochondria. CONCLUSION: Resvertrol has protective effects on focal cerebral ischemia-reperfusion injury via attenuating cerebral oxygen free radical lipid peroxidation and protecting mitochondria.