| 醋酸钠-醋酸体系DNA、组蛋白复合产生共振光散射衰减测定铝 |
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| 引用本文： | 刘凤海,杜晓燕,郑晶,吕昌武,肖颖,陈卫东. 醋酸钠-醋酸体系DNA、组蛋白复合产生共振光散射衰减测定铝[J]. 中国卫生检验杂志, 2005, 15(6): 646-647 |
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| 作者姓名： | 刘凤海 杜晓燕 郑晶 吕昌武 肖颖 陈卫东 |
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| 作者单位： | 1. 黑龙江省牡丹江市疾病预防控制中心,黑龙江牡丹江,157011
2. 哈尔滨医科大学公共卫生学院,哈尔滨,150000 |
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| 基金项目： | 黑龙江省科学技术计划项目(20020101) |
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| 摘 要： | 目的：探讨微量铝的测定方法。方法：共振光散射衰减法。结果：在pH值5．5,0．25mg／LDNA及40mg／L组蛋白条件下，扫描光谱显示最大光散射波长为332．8nm，铝离子浓度在0．54-4．86mg／L范围内，和光散射强度呈负相关，线性回归方程为y=10．9-0．0189X，回归系数为0．998，回收率在92％-106％。结论：该方法灵敏、简便、快速，适合进行微量铝离子的测定。
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| 关 键 词： | 共振光散射 组蛋白 衰减 DNA 醋酸钠 复合 线性回归方程 测定方法 离子浓度 散射强度 回归系数 微量铝 pH值 负相关 回收率 铝离子 |
| 文章编号： | 1004-8685(2005)06-0646-02 |
| 修稿时间： | 2005-02-28 |
The determination of aluminum based on light-scattering decreased in sodium acetate-acetate buffer solution,DNA and histone system |
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| Liu Feng-hai,DU Xiao-yan,Zheng Jing,Lv Chang-wu,Xiao Ying,Chen Wei-Dong. The determination of aluminum based on light-scattering decreased in sodium acetate-acetate buffer solution,DNA and histone system[J]. Chinses Journal of Health Laboratory Technology, 2005, 15(6): 646-647 |
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| Authors: | Liu Feng-hai DU Xiao-yan Zheng Jing Lv Chang-wu Xiao Ying Chen Wei-Dong |
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| Affiliation: | Liu Feng-hai 1,Du Xiao-Yan 2,Zheng Jing 2,Lv Chang-wu 1,Xiao Ying 1,Chen Wei-Dong 1 |
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| Abstract: | Objective:To explore a method for the examination of tittle aluminum.Methods:The light-scattering decreased method.Results:If pH was 5.5,DNA was 0.25 mg/L and histone was 40 mg/L, scan spectrum showed that the maxmal light-scattering wavelength was 332.8 nm.There was a liner relationship between the quantity of aluminum and the intensity of light-scattering.The linear range were 0.54~ 4.86 mg/L and the equation were Y=10.9-0.0189X, the recovery rates were in the range 92%~106%.Conclusion:The method is sensitive,simple and quick, so it is reliable to measure the mount of aluminum. |
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| Keywords: | Acetate-acetate buffer solution Histone DNA Aluminum |
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