大鼠急性出血坏死性胰腺炎的内毒素血症与组织改变的关系及纳屈酮治疗作用

目的：应用牛磺胆酸钠诱发大鼠急性出血坏死性胰腺炎（ＡＨＮＰ）模型，探讨大鼠ＡＨＮＰ的内毒素血症与胰腺组织改变的关系及纳屈酮治疗作用。方法：实验分为三组：对照组，ＡＨＮＰ组及纳屈酮（ＮＴＸ）治疗组；以６、１２、２４为观测时点，观察三组大鼠血浆淀粉酶、内毒素、胰腺系数及胰腺组织变化。结果：发现对应各时点ＡＨＮＰ组较对照组的血浆淀粉酶、内毒素（ＥＴ）及胰腺系数升高，胰腺组织明显损害；纳屈酮（ＮＴＸ）治疗组各时点较ＡＨＮＰ组的血浆淀粉酶、ＥＴ及胰腺系数下降；而且ＮＴＸ组胰腺损害减轻。结论：ＮＴＸ可能通过降低血浆ＥＴ，而减轻胰腺损害，延长ＡＨＮＰ大鼠生存时间及降低病死率。

THE RELATIONSHIP BETWEEN ENDOTOXEMIA AND HISTOLOGIC CHANGES IN THE PANCREATIC TISSUE AND THERAPEUTIC EFFECTS OF NALTREXONE ON EXPERIMENTAL ACUTE HEMORRHAGIC NECROTIZING PANCREATITIS IN RATS

Objective: A model of acute hemorrhagic necrotizing pancreatitis(AHNP) was induced by retrograde injection of 5% sodium taurocholate into the pancreatic duct for exploring the relationship between endotoxemia and histologic changes in the pancreatic tissue and therapeutic effects of naltrexone on AHNP in rats. Methods: The rats were randomly divided into three groups: control group, AHNP group and NTX treatment group. We studied the effects of morphine receptor blocker, naltrexone during the rats of AHNP and measured the plasma levels of amylase, pancreatic index, endotoxin and calculated the death rate as well as average survival time in rats of both AHNP and NTX groups. Changes of the pancreatic tissue was examined by light and electric microscopy. Results: The results demonstrated that amylase, endotoxin were significant fall in the plasma; besides, the damage of pancreatic tissue was reduced. Conclusion: NTX decreased endotoxin in the plasma and improves the damage of pancreatic tissue. The leth ity rate in rats of AHNP was significantly lower in NTX treatment.