早期强制性使用对局灶性脑梗死大鼠大脑皮层神经细胞黏附因子表达的影响

目的:探讨早期强制性使用对局灶性脑梗死大鼠大脑皮层神经细胞黏附因子(NCAM)表达的影响。方法:70只Wistar大鼠随机分为假手术(D)组(10只)和手术组(60只),后者制作成右侧大脑中动脉阻塞(MCAO)模型24h后再分为患侧前肢固定(A)组、未固定(B)组和健侧前肢固定(C)组,每组20只,并进行肢体固定。21天后解除固定,于MCAO后21天和28天时,用免疫组化的方法检测梗死灶周围大脑皮质NCAM的表达。结果:与D组相比,手术组大鼠梗死灶周围皮质阳性神经元数量明显增多(P<0.01),染色明显增强(P<0.05或P<0.01);手术组大鼠梗死灶周围皮质阳性细胞数及光密度值,B组多于(高于)A组,A组多于(高于)C组(P<0.05)。结论:脑缺血梗死可诱发NCAM的表达,肢体固定或过度使用对梗死灶周围皮质部分神经元代偿性高表达NCAM有不利影响,其中患侧前肢过度使用的影响尤其明显。

Effects of early forced use on the expression of neural cell adhesion molecule in the peri-ischemia cortex after local cerebral infarction

Objective:To study the effect of early forced use on expression of NCAM in the peri-ischemia cortex after local cerebral infarction.Method: 70 rats were randomly assigned into: sham-operated group (group D n=10) and operated group (n=60). The 60 Wistar rats coagulated at the right middle cerebral artery were divided into 3 groups at random: cast of the anterior limb on the contralateral side(group A), no cast fixation(group B) and ipsilateral side cast (group C). The cast was used at 24 hours after MCAO and removed at day 21st postsurgery.The expression of NCAM in the peri-ischemia cortex of the three groups were evaluated at 21d and 28d after the infarction.Result:In operated group the number of NCAM labeled cells and the expression of NCAM increased significantly in comparison with group D(P<0.05,P<0.01).The number of NCAM labeled cells and the expression of NCAM in group B were higher than those in group A and group C(P<0.05,P<0.01);Those in group A were higher than those in group C(P<0.05).Conclusion: Cerebral infarction could induce the expression of NCAM.Immobilication of UE,especially immobilization of unaffected-UE (forced use affected-UE),inhibited expression of NCAM in the peri-ischemic cortex.