煅狗骨对实验性骨折愈合作用的垂体TSH,GH细胞免疫细胞化学研究

为检测煅狗骨促进骨折愈合过程中垂体前叶促甲状腺激素(TSH)细胞和生长激素(GH)细胞结构和功能变化,本实验选用家兔54只,制成双侧桡骨标准骨折模型,分成煅狗骨用药组和对照组,术后每周定期取材。脑垂体常规石蜡包埋切片,行免疫细胞化学染色。结果显示对照组骨折后垂体TSH细胞及GH细胞数量均减少,而煅狗骨用药组则并未出现对照组的抑制性变化,TSH细胞数量一直维持较高水平,尤其GH细胞数量随术后时间延长而大幅度上升,因而说明煅狗骨能促进垂体TSH、GH细胞的功能,有利于骨折的愈合。

A cellular immunocytochemical study on pituitary TSH and GH during experimental healing process treated with calcined dog bone

For determination of the cellular structual and functional changes of the anterior lobe of pituitary thyroid stimulating hormone and growth hormone during the course of promoting fracture healing ability of calcined dog bone, fifty four rabbits were selected to make models of standard fracture of bilateral radius. They were divided into calcined dog bone treating group and control group. Materials were taken for every week postoperatively. The pituitary glands were prepared with paraffin embedding and sections as routinely and stained with immunocytochemical method. The results showed that in control group after fracture, there were decreasing of amount of pituitary TSH and GH cells. There was no such inhibitory changes in the calcined dog bone group. The amount of TSH cellular maintained within a rather high level.Especially the GH cells were elevated in a large scale along with prolongation of the course postoperatively. So it indicates that the calcined dog bone bears the action of promoting the function of pituitary TSH and GH cells and facilitates bone healing.