| HPLC法测定狼牙刺种子中苦参碱和氧化苦参碱含量 |
| |
| 引用本文： | 江海,吴三桥,李新生,田姗.HPLC法测定狼牙刺种子中苦参碱和氧化苦参碱含量[J].齐鲁药事,2008,27(12). |
| |
| 作者姓名： | 江海 吴三桥 李新生 田姗 |
| |
| 作者单位： | 1. 陕西省资源生物重点实验室,陕西,汉中,723000
2. 汉中3201医院,陕西,汉中,723000 |
| |
| 基金项目： | 陕西省教育厅资助项目
，
陕西理工学院资助项目
|
| |
| 摘 要： | 目的建立分析测定狼牙刺中苦参碱和氧化苦参碱含量的高效液相色谱法。方法色谱柱为ZORBAX-C18柱(4.6mm×150mm,5μm);流动相:0.04 mol.L-1磷酸溶液(pH1.8)-甲醇(90∶10);流速1.0mL.min-1;检测波长220nm;柱温20℃;试样进样量10μL;检测时间12min。结果苦参碱、氧化苦参碱与其他组份均能达到基线分离。结论该方法能够分析测定狼牙刺中苦参碱和氧化苦参碱。
|
| 关 键 词： | 高效液相色谱法 苦参碱 氧化苦参碱 狼牙刺 |
Determination of matrine and oxymatrine in Sophora viciifolia Hance. by HPLC |
| |
| JIANG Hai,WU San-qiao,LI Xin-sheng,Tian Shan.Determination of matrine and oxymatrine in Sophora viciifolia Hance. by HPLC[J].qilu pharmaceutical affairs,2008,27(12). |
| |
| Authors: | JIANG Hai WU San-qiao LI Xin-sheng Tian Shan |
| |
| Institution: | JIANG Hai,WU San-qiao,LI Xin-sheng,Tian Shan1 |
| |
| Abstract: | OBJECTIVE To established an analytical method of matrine and oxymatrine in Sophora viciifolia Hance.by HPLC.METHODS ZORBAX-C18(150mm×4.6mm,5μm)column was used and the mobile phase was 0.04mol·L-1 H3PO4(pH 1.8)-CH3OH(90∶10).The flow rate was 1.0mL·min-1 and detection wavelength was 220nm.Column temperature was 20℃,injection volume was 10μL and lasted 12min.RESULTS Matrine,oxymatrine and other substance were separated in the method.CONCLUSION The method could determine matrine and oxymatrine in Sophora viciifolia. |
| |
| Keywords: | HPLC matrine oxymatrine Sophora Viciifolia Hance |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
