33批茶多酚高效液相色谱指纹图谱的质量控制研究

 目的建立茶多酚的数字化色谱指纹谱,结合主要单体成分的含量检测,作为茶多酚质量控制的依据。方法采用Supelco Discovery C₁₈色谱柱,含0.1％甲酸的水-甲醇梯度洗脱的HPLC色谱系统,检测波长210 nm。结果依照33个批次茶多酚样品的色谱和光谱数据构建起色谱指纹谱,各项方法学考察结果良好。茶多酚产品的特征指纹检出率应在80％以上,咖啡因的相对峰面积值(100 Ar)不应超过5。结论能科学地、客观公正地反映样品的真实组成和内在品质。

Study on HPLC-Fingerprint Quality Control of Thirty-Three Batches of Tea Polyphenols

OBJECTIVE To establish the digital fingerprint spectrum(FPS)of tea polyphenols, combining with the determination of major components, as a new mode to evaluate and control the quality of tea polyphenols.METHODS The separation was performed on a C18 column with a methanol-water-0.1% formic acid gradient elution system, the detection wavelength was at 210 nm.RESULTS Thirty-three batches of tea polyphenols samples were analyzed by the HPLC-DAD chromatographic system and FPS was established according to the obtained data. The analytical results of methodologic experiments were favorable. CONCLUSION The FPS of tea polyphenols is accurate and feasible, it reflects the real constitution and inherent quality of samples. The FPS of tea polyphenols is suitable for the quality control. The detection ratio of characteristic peaks in tea polyphenols samples should be above 80%, and the relative area (magnified one hundred times) of caffeine should be lower than five.