脐血S-100B蛋白在新生儿缺氧缺血性脑病早期诊断中的价值

目的 通过观察S-100B蛋白在新生儿缺氧缺血性脑病(HIE)患儿脐血中的变化,探讨其在HIE早期诊断中的作用.方法 选择足月窒息新生儿307例为观察对象,其中符合HIE诊断标准的46例纳入HIE组.HIE组中轻度HIE 31例,中、重度15例.同期分娩的正常足月新生儿43例为正常对照组.分别留取脐血.采用双抗体夹心ELISA方法检测脐血血清S-100B蛋白浓度.结果 ①在正常组内男性与女性脐血血清S-100B无差异.②正常组脐血血清S-100B蛋白浓度为(1.03±0.32)μg/L,HIE组为(2.53±1.10)μg/L,2组比较差异有统计学意义(t=8.848,P＜0.05).③轻度HIE组与中、重度HIE组脐血S-100B蛋白浓度分别为(2.06±0.65)μg/L与(3.49±1.23)μg/L,与正常组比较差异有统计学意义(F=79.6909,P＜0.01).结论 性别对新生儿脐血S-100B无影响.脐血S-100B可作为HIE的早期生化指标,且可以判断脑损伤程度.

Serum S-100B concentration of umbilical cord blood in the early diagnosis of newborn hypoxic ischemic encephalopathy

Objective To investigate the role of serum S-100B of umbilical cord blood in the early diagnosis of newborn hypoxic ischemic encephalopathy(HIE).Methotis 307 full-term newborns with asphyxia were selected as subiects and 43 normal full-term newborns delivered during the corresponding period were selected as controls.The umbilical cord blood sample was acquired.46 cases of 307 subjects with asphyxia developed into HIE,31 cases with mild HIE and 15 cases with moderate and severe HIE.The serum S-100B concentration of umbilical cord blood was detected by ELISA analysis.Results ①There was no significant difference in the serum S-100B concentration of umbilical cord blood between the male sub-group and female sub-group in normal group;②The concentration in control group and HIE group were(1.03±0.32)μg/L and(2.53±1.1)μg/L respectively(t=8.848,P＜0.05＝;③The concentration in mild HIE group and moderate to severe HIE group were(1.03±0.32)μg/L nd(3.49±1.23)μg/L respectively,which were significantly different with control group(F=79.6909,P＜0.01).Conclusion Sex has no significant relation to the serum S100B concentration.Serum S-100B concentration of umbilical cord blood can be biomarkers for early diagnosis of newborn HIE.