人参皂苷Rg3对大鼠子宫内膜异位症组织中血管内皮生成因子影响的研究

目的 观察人参皂苷Rg3（Rg3）对大鼠子宫内膜异位症（endometriosis，EMs）组织中血管内皮生成因子（vascular endothelial growth factors，VEGF）影响和抗血管生成作用。方法 参照Jones方法建立Wistar大鼠EMs动物模型，3周后将建模成功的大鼠随机分为空白对照、孕三烯酮和Rg3各2．4，6及8周组,共12组。用SABC免疫组化法测定异位灶组织中VEGF的表达。结果 Rg3治疗大鼠EMs模型2，4，6，8周后，异位组织中VEGF的表达明显低于对照组，差异有显著意义（P〈0．05）；而孕三烯酮治疗相同时间后与对照组相比，VEGF表达无明显改变，差异无显著意义（P〉0．05）；Rg3组随治疗时间延长，VEGF明显减少（P〈0．05），而对照组和孕三烯酮组无此表现。结论 Rg3对大鼠子宫内膜异位组织中VEGF表达和新生血管生成均有抑制作用。Rg3有望成为子宫内膜异位抗血管生成治疗新药。

The study of ginsenoside Rg3 on the expression of vascular endothelial growth factors in ectopic endometrium of rats

Objective To observe the effect of ginsenoside Rg3 on the expression of vascular endothelial growth factors(VEGF) and antiangiogenesis in ectopic endometrium of rats. Methods Wistar rats endometriosis models were established referring to Jones methods, the model rats were divided into the control group, ginsenoside Rg3 group, gestrinone group, each group were divided into 2 weeks, 4 weeks, 6 weeks and 8 weeks. VEGF was observed in ectopic endometrium by immunohistochemistry (SABC technique). Results VEGF in ectopic endometrium were decreased remarkably after treatment of ginsenoside Rg3 after 2,4,6,8 weeks (P<0. 05), while the difference was not significant (P>0. 05) in the gestrinone group. And with the time prolonging, the expression of VEGF was reduced remarkably(P <0. 05) while the change was not observed in the control group and the gestrinone group. Conclusion Ginsenoside Rg3 has the effect on inhibiting the expression of VEGF and angiogenesis in ectopic endometrium of rats. Ginsenoside Rg3 maybe a new drug of antiangiogenesis in ectopic endometrium.