高+Gx对猴肝细胞c-fos表达的影响

目的:观察航天应急返回过程中高正加速度( Gx)对肝脏细胞c-fos基因表达的影响．方法:选用♂猕猴(共9只)为对象,随机分为4组,对照组承受 1Gx,300 s的超重作用;实验组根据承受过载峰值的大小分为3个亚组,其承受过载峰值分别为 15Gx,200 s; 18Gx, 165 s; 21Gx,140 s．观察高 Gx对猴肝脏细胞c-fos基因表达的影响．结果:实验组肝脏细胞胞质呈现不同程度的水肿及泡状变性,c-fos基因表达明显增强,呈弥漫性细胞质内棕黄色着色;肝细胞c-fos基因表达程度随超重剂量的增加有增强趋势．对照组肝脏组织病理学改变程度明显较实验组轻微,c-fos基因表达亦明显减弱．结论: Gx可引起猴肝脏组织细胞c-fos基因表达增强,提示有早期肝脏组织损伤．

Effect of hypergravity on expression of c-fos gene in hepatocytes of Rhesus macaque

AIM: To detect the expression of c-fos in the hepatocytes of Rhesus macaque under hypergravity ( Gx) loads. METHODS: A total of 9 male Rhesus macaques were randomly divided into experiment groups (n = 7) and control group (n = 2). The animals in control group was exposed to lGx/300s overloads, and those in the experimental groups were exposed to the overloads of 15Gx/200s (n = 2), 21Gx/165s (n = 2) and 21Gx/140s (n = 3), respectively. Liver tissue was fixed with 40 g/L buffered formaldehyde and embedded with paraffin, and histopathological changes were observed under microscope. The expression of c-fos was detected by an immunohistochemical method. RESULTS: In the experimental groups, cytoplasmic swelling and alveolar degeneration were found in the liver; the expression of c-fos was detected in the cytoplasm of hepatocytes, with a tendency of enhancement as the dosage of hyper-gravity increased. In the control group, no significant histopathological changes were observed and the expression of c-fos was also remarkably decreased. CONCLUSION: Overload of gravity can induce significant injuries of the liver in Rhesus macaque.