| 中国人磷脂转运蛋白基因克隆及序列分析 |
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| 引用本文： | 马百坤,庄一义,李克,吴向东.中国人磷脂转运蛋白基因克隆及序列分析[J].中国动脉硬化杂志,2002,10(1):48-50. |
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| 作者姓名： | 马百坤 庄一义 李克 吴向东 |
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| 作者单位： | 南京军区南京总医院医学检验中心,江苏,南京,210002 |
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| 摘 要： | 为探讨中国人磷脂转运蛋白的基因结构、组成及特点 ,克隆了中国人磷脂转运蛋白基因。首先提取人胎肝组织总RNA ,以此为模板利用逆转录—聚合酶链反应法成功钓取了磷脂转运蛋白成熟肽基因片段 ,DNA序列分析所得基因片段由 14 2 8个碱基组成
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| 关 键 词： | 磷脂转运蛋白 基因克隆 逆转录 聚合酶链反应 中国人 |
| 文章编号： | 1007-3949(2002)10-01-0048-03 |
| 收稿时间： | 2001/6/27 0:00:00 |
| 修稿时间： | 2001年6月27日 |
Molecular Cloning and Sequencing of Chinese Phospholipicl Transfer Protein Gene |
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| MA Bai Kun,ZHUANG Yi Yi,LI Ke,and WU Xiang Dong.Molecular Cloning and Sequencing of Chinese Phospholipicl Transfer Protein Gene[J].Chinese Journal of Arteriosclerosis,2002,10(1):48-50. |
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| Authors: | MA Bai Kun ZHUANG Yi Yi LI Ke and WU Xiang Dong |
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| Institution: | Center of Medical Laboratory Sciences of PLA, Jinling Hospital, Nanjing 210002, China |
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| Abstract: | Aim To identify, clone and sequence Chinese ma ture Phospholipicl Transfer Protein (PLTP) peptide gene. Methods Total RNA was extracted from Chinese fetal liver tissue, cDNA fragment encoding human PLTP was amplified by RT PCR using specific primers, and then was cloned into pGEM T vector. Inserted PLTP gene was sequenced by ABI 377 DNA Sequenc er. Results Chinese PLTP gene was successfully cloned. Conclusion Analytical result indicates it is 1 428 b ase pairs in length and has 100% nucleotide homology with that reported previous ly. |
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| Keywords: | Phospholipid Transfer Protein Cloning RT PCR Chinese |
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