非小细胞肺癌组织MIP-1表达及其与树突状细胞浸润的关系

目的探讨非小细胞肺癌组织MIP-1表达及其与树突状细胞浸润的关系。方法选择74例非小细胞肺癌组织标本,采用荧光原位杂交法检测MIP-1的表达,免疫组织化学检测趋化因子受体CCR1、CCR7的表达。结果肺癌细胞胞质及胞膜表达MIP-1,同时检测到肺癌组织中有CCR1和CCR7阳性树突状细胞的浸润。MIP-1、CCR1和CCR7阳性树突状细胞的表达和肺癌分期相关。肺癌组织中MIP-1阳性细胞表达率与CCR1阳性树突状细胞数量呈正相关,而与CCR7阳性树突状细胞数量呈负相关。结论非小细胞肺癌组织MIP-1表达与肿瘤浸润树突状细胞表面分子CCR1、CCR7的表达关系密切。

Expression of MIP-1 in non-small cell lung cancer and its relationship with dendritic cell infiltration

Objective To explore the expression of MIP-1 in non-small cell lung cancer（NSCLC） and its relationship with dendritic cell infiltration.Methods Immunohistochemical staining was performed to detect expressions of CCR1 and CCR7,and fluorescence in situ hybridization was performed to detect the expression of MIP-1 in 74 lung cancer tissues.Results Both MIP-1 positive tumor cells and both CCR1 and CCR7 positive dendritic cells were detected in lung cancer tissues.The expressions of MIP-1,CCR1 and CCR7 positive cells were related to lung cancer staging.The expression levels of MIP-1 in lung cancer were in positive correlation with the percentage of CCR1 positive dendritic cells and had a negative correlation with the percentage of CCR7 positive dendritic cells.Conclusion The expression of MIP-1 is closely related to expressions of CCR1 and CCR7 from tumor infiltrating dendritic cells.