双特异性单抗对胃肠癌的体内杀伤作用

目的探讨抗CD3抗CEA双特异性单链抗体介导CIK细胞在体内杀伤胃肠癌的作用。方法分别将人胃低分化腺癌BGC823细胞和人结肠中分化腺癌SW1116细胞种植裸鼠后,建立裸鼠胃癌及肠癌模型,分为3组分别经尾静脉注入生理盐水、CIK细胞及CIK细胞＋双特异性单抗,每3天治疗1次,共6次,取出肿瘤组织称重,并计算出各组的抑瘤率。结果在体内,CIK组和CIK＋双抗组均可抑制肿瘤生长,对胃癌的抑瘤率分别为29.90%和44.33%。对结肠癌的抑瘤率分别为14.93%和38.81%。CIK＋双抗组：胃癌及结肠癌的瘤体积、瘤重明显小于CIK组。结论 CIK细胞本身可以抑制肿瘤细胞的生长,在加入抗CD3抗CEA双特异性单链抗体后抑瘤作用明显增强。

In vivo killing effect of bispecific single-chain antibody on gastric and colon cancer cells

Objective To investigate the killing effect of CIK cells mediated by bispecific single-chain antibody,anti-CD3 and anti-CEA,on gastric and colon cancer cells in vivo.Methods Gastric and colon cancer models in nude mice were established after BGC823 cells from human poorly differentiated gastric adenocarcinoma and SW1116 cells from human moderately differentiated colon adenocarcinoma were planted into nude mice.The mice were divided into 3 groups,which were injected with normal saline,CIK cells and CIK cells plus bispecific antibody,respectively,once every 3 days for 6 times.Then the tumors were removed and weighed,and the inhibition rate in each group was calculated.Results Both the CIK cells and CIK cells plus bispecific single-chain antibody could inhibit tumor growth.Inhibition rates of gastric cancer were 29.90% and 44.33% respectively.Inhibition rates of colon cancer were 14.93% and 38.81% respectively.Compared with CIK cell group,the size and weight of the tumor were significantly smaller in CIK cells plus bispecific singlechain antibody group.Conclusion CIK cells alone can inhibit tumor cell growth and combination of CIK cells and the bispecific single-chain antibody,anti-CD3 and anti-CEA,is more effective in inhibiting tumor growth.