诱导痰细胞和白细胞介素8 白细胞介素9 嗜酸性粒细胞趋化因子在儿童哮喘发病中的作用

探讨诱导痰细胞和白细胞介素8(IL 8)、白细胞介素9(IL 9)、嗜酸性粒细胞趋化因子(eotaxin)在儿童哮喘发病中的作用及相互关系。 方法以2004年9月至2005年3月重庆医科大学附属儿童医院确诊哮喘的44例患儿为研究对象，其中急性发作期25例、缓解期19例，同时选10例因骨骼畸形入院的患儿作对照组，进行诱导痰的细胞分类检查和ELISA方法检测IL 8、IL 9、eotaxin。 结果哮喘患儿诱导痰细胞总数明显升高，急性发作期诱导痰中性粒细胞数明显高于缓解期和对照组，缓解期淋巴细胞数明显高于急性发作期和对照组，嗜酸性粒细胞各组间比较差异无显著性意义；哮喘患儿诱导痰IL 8、IL 9质量浓度与对照组比较有明显升高，且IL 8、IL 9质量浓度与中性粒细胞百分数呈正相关，eotaxin在各组间比较差异无显著性意义。 结论与成人过敏性哮喘不同，中性粒细胞在儿童哮喘的发病中扮演重要角色，IL 8趋化中性粒细胞聚集于气道，中性粒细胞在IL 9的作用下产生和释放IL 8，三者相互影响，共同参与哮喘的发病。

The role of cells,interleukin 8,interleukin 9 and eotaxin in induced sputum in childhood asthma.

AbstractObjectiveTo investigate the cells,interleukin 8,interleukin 9 and eotaxin roles in induced sputum of asthmatic children. MethodsFrom Sep.2004 to Mar.2005,44 asthmatic children(including 25 exacerbation,19 remission) and 10 normal children were selected to induce sputum by hypertonic saline in the Affiliated Children's Hospital of Chongqing University of Medical Sciences.Sputum was processed and cytospin was examined for a differential cell count.The levels of IL 8,IL 9 and eotaxin in sputum supernatant were detected by ELISA. ResultsThere were more cells in asthmatic children induced sputum than in normal children.The number of sputum neutrophil was higher in exacerbation children than in remission children and normal children.The number of sputum lymphocyte was higher in remission children than in exacerbation and normal children.The difference of sputum eosinophil was not found in each group.The levels of sputum supernatant IL 8 and IL 9 were significantly higher in asthmatic children than in normal children.A significant positive correlation between the levels of IL 8 and neutrophil,IL 9 and neutrophil was found.There was no difference in the sputum supernatant eotaxin level in each group. ConclusionUnlike adult allergic asthma,neutrophil plays a critical role in childhood asthma.Interaction of neutrophil,IL 8 and IL 9 secretion attributes to the pathogenesis of childhood asthma.