| 单克隆抗体F36/22分离和检测小鼠移植性乳腺肿瘤抗原的研究 |
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| 引用本文： | 蔡桂英. 单克隆抗体F36/22分离和检测小鼠移植性乳腺肿瘤抗原的研究[J]. 四川大学学报(医学版), 1990, 0(1) |
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| 作者姓名： | 蔡桂英 |
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| 作者单位： | 华西医大基础医学院生物化学教研室 |
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| 摘 要： | 作者应用免疫印迹法检测到单克隆抗体F36/22能识别小鼠移植性乳腺癌抗原.用SepharoseCL-4BA单克隆抗体F36/22免疫亲和层析法分离纯化了BALB/c小鼠移植性乳腺肿瘤抗原,固相放射免疫法检测纯化188.4倍,SDS-PAGE及免疫印迹检测其分子量为60kd。
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| 关 键 词： | 乳腺肿瘤抗原 单克隆抗体 免疫印迹法 亲和层析 固相放射免疫法 小鼠 |
Isolation and Detection of Murine Transplantable Breast Tumor Antigen by McAb F36/22 |
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| Cai Guiying. Isolation and Detection of Murine Transplantable Breast Tumor Antigen by McAb F36/22[J]. Journal of Sichuan University. Medical science edition, 1990, 0(1) |
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| Authors: | Cai Guiying |
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| Affiliation: | Cai Guiying Department of Biochemistry |
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| Abstract: | A transplantable murine breast tumorantigen (called JC tumor) has been recog-nized by the detection of McAb F36/22 withthe method of immunoblotting. JC tumorantigen was isolated by Sepharose-CL-4B,then was purified by McAb F36/22 affinitychromatography. Solid phase RIA detectiondemonstrated that the specific activity ofthe antigen which was purified by 188.4times was 148000 cpm/mg protein. SDS-PAGE and immunoblot analysis revealedthat the tumor antigen possessed a molecularweigh of 60kd. Competitive binding RIAindicated that JC tumor antigen inhibitedthe combining of ductal carcinoma antigenwith McAb F36/22. It implied that somegroups (antigen determinant)on JC tumorantigen were the same as or similar tothose on ductal cancer antigen. |
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| Keywords: | Breast tumor antigen Affinity chromatography Immunoblot Monoclonal antibodies Solid phase radio-immunoassay |
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