亲和吸附材料特异性清除肠源性内毒素有效性和安全性探讨

目的 探究本课题组研发的亲和吸附材料特异清除肠源性内毒素的有效性及安全性?方法 运用阑尾结扎穿孔的方法建立犬肠源性内毒素血症模型,进行灌流治疗?实验过程中选取多个时间点, 监测生命体征,采血检测内毒素含量及血液生理生化指标?结果 灌流吸附1?2 h 后内毒素含量为(0.49 ± 0.22)?(0.034 ±0.00)Eu/ mL,与灌流开始(7.25 ± 1.18) Eu/ mL 相比,差异有显著性( P < 0.05)?灌流2 h 后平均清除率R =99.52%?灌注治疗后白细胞?红细胞?血红蛋白?血小板?总蛋白?白蛋白?球蛋白?谷丙转氨酶?谷草转氨酶?尿素?肌酐指标均有所下降,与灌流开始相比差异有显著性( P <0.05),各值均在正常值范围?灌流过程中犬的生命体征平稳?结论 本课题研发的亲和吸附材料能高效清除实验犬血液中内毒素并有良好的血液相容性?

Efficacy and safety of the removal of intestinal endotoxin in the blood by affinity adsorption materials

Objective To investigate the efficacy and safety of the affinity adsorption material for removal of intestinal endotoxin in the blood. Metheds To establish a canine model of intestine-derived endotoxemia by ligation and perforation of the appendix. Hemoperfusion was performed to treat the endotoxemia in model dogs. In the experiment, we monitored vital signs and detect the content of endotoxin and blood physiological and biochemical indexes at different time points. Results The endotoxin content was (0.49 ± 0.22), (0.034 ± 0.00) Eu/ mL after hemoperfusion for 1 and 2 hours, compared with that before the beginning of perfusion(7.25 ±1.18)Eu/ mL, showing a significant difference ( P <0.05). After treatment for 2 hours, the average clearance rate was 99.52%. White blood cells, red blood cells, hemoglobin, platelets, total protein, albumin, globulin, alanine aminotransferase, aspartate aminotransferase, urea and creatinine index were significantly decreased after perfusion ( P < 0.05). The index values were in a normal range and vital signs were stable during the perfusion. Conclusions The affinity adsorption material developed by our team can effectively remove endotoxin in the blood of experimental dogs and has good blood compatibility.