Effect of ACEA—a selective cannabinoid CB1 receptor agonist on the protective action of different antiepileptic drugs in the mouse pentylenetetrazole-induced seizure model |
| |
Authors: | Marta Andres-Mach Dorota Zolkowska Beata Barcicka-Klosowska Agnieszka Haratym-Maj Magdalena Florek-Luszczki Jarogniew J Luszczki |
| |
Institution: | 1. Isobolographic Analysis Laboratory, Institute of Rural Health, Jaczewskiego 2, PL 20–950 Lublin, Poland;2. Department of Neurology, UC Davis School of Medicine, 4635 2nd Avenue, Sacramento, CA 95817, USA;3. Department of Pathophysiology, Medical University of Lublin, Jaczewskiego 8, PL 20–090 Lublin, Poland;4. Department of Physiopathology, Institute of Rural Health, Jaczewskiego 2, PL 20–950 Lublin, Poland;5. Department of Public Health, Institute of Rural Health, Jaczewskiego 2, PL 20–950 Lublin, Poland |
| |
Abstract: | Endogenous cannabinoid ligands and cannabinoid CB1 receptor agonists have been shown to exert anticonvulsant effects in various experimental models of epilepsy. The purpose of this study was to determine the effects of arachidonyl-2′-chloroethylamide (ACEA—a highly selective cannabinoid CB1 receptor agonist) on the protective action of clonazepam, ethosuximide, phenobarbital, and valproate against pentylenetetrazole (PTZ)-induced clonic seizures in mice. To ascertain any pharmacokinetic contribution of ACEA to the observed interactions between tested drugs, free (non-protein bound) plasma and total brain concentrations of the antiepileptic drugs were estimated. Additionally, acute adverse-effect profiles of the combination of ACEA and different classical antiepileptic drugs (clonazepam, ethosuximide, phenobarbital and valproate) with respect to motor performance, long-term memory and skeletal muscular strength were measured. |
| |
Keywords: | ACEA arachidonyl-2&prime -chloroethylamide ACPA arachidonyl-cyclopropylamide AEDs antiepileptic drugs NO nitric oxide PMSF phenylmethylsulfonyl fluoride WIN WIN 55 212-2 mesylate |
本文献已被 ScienceDirect 等数据库收录! |
|