Spirulina or dandelion-enriched diet of mothers alleviates lead-induced damages in brain and cerebellum of newborn rats |
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Authors: | Manel Gargouri,Fatma Ghorbel-Koubaa,Michè le Bonenfant-Magné ,Christian Magné ,Xavier Dauvergne,Riadh Ksouri,Yousef Krichen,Chedly Abdelly,Abdelfattah El Feki |
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Affiliation: | 1. Laboratoire d’Ecophysiologie Animale, Faculté des Sciences de Sfax, Route de Soukra, 3038 Sfax, Tunisie;2. EA 2219 Geoarchi, UFR Sciences et Techniques, Université de Bretagne Occidentale, 6 Avenue V. Le Gorgeu, CS 93837, 29238 Brest Cedex 3, France;3. Laboratoire d’Adaptation des Plantes aux Stress Abiotiques, Centre de Biotechnologie de Borj- Cedria, BP 901, 2050 Hammam Lif, Tunisie;4. Ferme Marine Bio Algues Tunisie, BP 51, 5180 Ksour Essaf, Tunisie |
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Abstract: | This study was aimed at evaluating the toxic effects of a prenatal exposure to lead acetate on brain tissues of newborn rats, and potent protective effects of spirulina (Arthropira platensis) or dandelion (Taraxacum officinalis) added to rat diet. Female rats were given a normal diet (control) or a diet enriched with spirulina or dandelion. Additionally, lead acetate was administered to one half of these rats through drinking water from the 5th day of gestation, to day 14 postpartum. Lead toxicity was assessed by measuring blood lead levels, brain weight, tissue damage, as well as protein content, lipid peroxidation and activities of antioxidant enzymes in brain tissues of neonates. Lead poisoning of mothers caused lead deposition in the brain and cerebellum of newborns and cerebellum tissue damages. Moreover, a significant decrease in weight and protein content of these tissues was found. Oxidative stress and changes in antioxidant enzyme activities in brain tissues were also recorded. Conversely, no such damages or biochemical changes were found in neonates from plant fed lead-poisoned mothers. These results strongly suggest that beneficial effects of spirulina- or dandelion-added diet on lead-intoxicated rats proceeded through the reduction of the lead-induced oxidative stress and related damages. |
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Keywords: | CAT, catalase GPX, glutathione peroxidase ROS, reactive oxygen species SOD, superoxide dismutase TBARS, thiobarbituric acid reactive substances TBS, tris-buffer saline |
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