| PROTECTIVE EFFECT OF MELATONIN ON NEURAL CELLS AGAINST THE CYTOTOXICITY OF OXYRADICALS |
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| 引用本文： | 安沂华 刘恩重 刘相轸 杨富明 韩风平 戴钦舜. PROTECTIVE EFFECT OF MELATONIN ON NEURAL CELLS AGAINST THE CYTOTOXICITY OF OXYRADICALS[J]. 中国医学科学杂志(英文版), 2000, 15(1): 40-44 |
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| 作者姓名： | 安沂华 刘恩重 刘相轸 杨富明 韩风平 戴钦舜 |
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| 作者单位： | DepartmentofNeurosurgery,theFirstAffiliatedHospitalofHarbinMedicalUniversity,Harbin150001 |
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| 基金项目： | Korea Science & Engineering Foundation (KOSEF);; |
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| 摘 要： | Objective. To investigate the exact mechanism of melatonin to prohibit the apoptosis of neural cells induced by various kinds of cytotoxic agents. Methods. We used the methods of phase contrast microscopy, MTT assay and hoechst dye staining to check this mechanism in SKNSH and U251 cell lines. Results. Both 2mmol/L H2O2 and 0. 5 μmol/L amyloid β- protein (Aβ) induce these two cell lines die via apoptosis. Either melatonin or glutathione can significantly protect both cell lines. The protective effect of 10 μmol/L melatonin is as same as that of 60μmol/L glutathione. Conclusion. Melatonin can partly inhibit the cytotoxicity of H2O2 and Aβ through its role as a free radical scavenger.
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| 关 键 词： | 褪黑激素 保护作用 神经细胞 细胞毒素 |
PROTECTIVE EFFECT OF MELATONIN ON NEURAL CELLS AGAINST THE CYTOTOXICITY OF OXYRADICALS |
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| Yang Fuming,Han Fengping,Dai Qinshun. PROTECTIVE EFFECT OF MELATONIN ON NEURAL CELLS AGAINST THE CYTOTOXICITY OF OXYRADICALS[J]. Chinese medical sciences journal, 2000, 15(1): 40-44 |
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| Authors: | Yang Fuming Han Fengping Dai Qinshun |
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| Affiliation: | Department of Neurosurgery, First Affiliated Hospital of Harbin Medical University, Harbin 150001. |
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| Abstract: | OBJECTIVE: To investigate the exact mechanism of melatonin to prohibit the apoptosis of neural cells induced by various kinds of cytotoxic agents. METHODS: We used the methods of phase contrast microscopy, MTT assay and hoechst dye staining to check this mechanism in SKNSH and U251 cell lines. RESULTS: Both 2 mmol/L H2O2 and 0.5 micromol/L amyloid beta-protein (Abeta) induce these two cell lines die via apoptosis. Either melatonin or glutathione can significantly protect both cell lines. The protective effect of 10 micromol/L melatonin is as same as that of 60 micromol/L glutathione. CONCLUSION: Melatonin can partly inhibit the cytotoxicity of H2O2 and Abeta through its role as a free radical scavenger. |
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