红景天苷对小鼠帕金森模型的保护作用及机制

目的:研究红景天苷对百草枯(paraquat,PQ)所诱导的帕金森病(Parkinson's disease,PD)小鼠模型的神经保护作用及其机制.方法:采用雄性ICR小鼠腹腔注射百草枯制成PD小鼠模型,给予红景天苷治疗28 d后,观察各组PD小鼠行为学改变情况,用HPLC-EC法测定纹状体中DA和DOPAC含量;用酶联免疫吸附法(ELISA)测定小鼠脑中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)的含量;用蛋白免疫印记法(Western blot)检测脑中Rho、ROCKⅡ和NF-κBp65的蛋白表达.结果:经过红景天苷的治疗后,PD小鼠均有不同程度的神经行为学改善,显著减少PD小鼠的旋转圈数,自主活动增加.明显增加脑内纹状体中DA和DOPAC含量,明显降低百草枯引起的脑内IL-1β、IL-6、TNF-α含量.Rho,ROCKⅡ和NF-κB p65蛋白含量较模型组减少.结论:红景天苷对百草枯所诱导的PD小鼠的神经行为学、神经炎症反应有改善作用,可能通过Rho/ROCKⅡ通路调节NF-κB来抑制神经炎症,从而能够缓解帕金森病的进展.

Effects of Salidroside in Parkinson Disease Mice

Objective: The current study was designed to investigate the neuroprotective role of salidroside and its mechanism in Parkinson's disease (PD) mice. Methods: Paraquat was used to induce PD in mice. After 28 days of treatment with salidroside, the behavior changes were observed and in the striatum, DA and DOPAC were detected by HPLC-EC, interleukin 1 beta (IL-1β), IL-6 and tumor necrosis factor alpha were detected by ELISA and the levels of Rho, ROCKⅡand NF-κB p65 protein expression were detected by Western Blot. Results: Salidroside obviously alleviated the behavior changes in PD mice and increased the DA and DOPAC, reduced the IL-1β, IL -6 and tumor necrosis factor alpha and decreased the levels of Rho, ROCKⅡand NF-kappa Bp65 protein expression in the striatum. Conclusions: Salidroside alleviated paraquat-induced PD in mice and the effects may exert through the Rho/ROCKⅡ/NF-kB signaling pathway.