高原地区55型腺病毒的病原分离及全基因序列分析

目的 探讨高原地区55型腺病毒(HAdV-B55)基因变异特点.方法 采集驻西藏某部队55型腺病毒感染者咽拭子标本,采用HEp-2分离病毒株,通过分片段PCR扩增和测序获得HAdV-B55毒株的全基因序列,通过与内地毒株进行序列比对,发现其变异特点.结果 从55型腺病毒感染者咽拭子标本分离得到HAdV-B55毒株,命名为LS89/Tibet/2016,通过PCR产物测序拼接,获得了本次流行的HAdV-B55毒株的全基因序列,GenBank登录号为KY002683.通过MEGA6软件比对,发现该病毒基因序列并未发生大片段重组,与内地以往流行毒株(QS-DLL株)序列相似性为99.9%.结论 本次分离出的病毒基因序列未发生大片段重组.本研究为揭示55型腺病毒的进化特点及今后高原地区制订疾病防控策略提供了依据.

Pathogen isolation and whole-genome sequence analysis of human adenovirus type 55 outbreak on plateaus

Objective To investigate the variation characteristics of adenovirus type 55 (HAdV-B55) gene on plateaus.Methods Throat swabs were collected from HAdV-B55 infected patients and used for virus isolation in HEp-2 cells.The whole-genome sequence was obtained by PCR and sequencing.HAdV-B55 gene sequence was blast with the previously reported virus.Results HAdV-B55 strains were isolated from throat swabs, which were named LS89/Tibet/2016.The whole-genome sequence was obtained and submitted to GenBank with the accession number of KY002683.No large fragment gene recombination was found between this HAdV-B55 strain and previous strains, and the sequence similarity with QS-DLL strain was 99.9%.Conclusion This study provides more information for the evolution patterns of adenovirus 55 and will contribute to the prevention and control of HAdV-B55 infection in the future.