Gab2对结肠癌细胞细胞外黏附作用的影响

;目的探讨Gab2对结肠癌细胞外黏附作用的影响。方法选取SW620和HCT116两种结肠癌细胞系,建立Gab2表达降低和Gab2表达升高的稳定细胞株。将实验NOD/SCID小鼠分为3组,每组10只。(1)对照组;接种scr/MGC803体结肠癌细胞;(2)Gab2降低组;接种Gab2降低的siGab2/MGC803结肠癌细胞;(3)Gab2升高组;接种Gab2升高的Gab2/MGC803结肠癌细胞。检测细胞迁移能力,测量肿瘤的大小和侵袭范围及细胞凋亡情况。结果处理后3组细胞迁移数计算结果显示,Gab2降低组的细胞迁移数显著低于Gab2升高组和对照组(P<0.05)。各组NOD/SCID小鼠均在接种40 d后处死,比较接种前与接种后小鼠体质量,Gab2降低组显著低于Gab2升高组、对照组,对照组低于Gab2升高组。测量瘤体积,Gab2升高组高于Gab2降低组、对照组。Gab2降低组瘤体积抑制率显著高于对照组(P<0.05)。相关分析发现,MMP-2、MMP-9蛋白的表达与Gab2蛋白表达呈正相关。结论 Gab2可有效调控体外细胞迁移数,显著抑制小鼠结肠癌细胞侵袭及转移,通过调节MMP-2、MMP-9蛋白表达,从而抑制结肠癌生长及转移。

Effect of Gab2 on extracellular adhesion of colon cancer cells

Objective To investigate the effect of Gab2 on extracellular adhesion of colon cancer cells.Methods SW620 and HCT116 were selected to establish the stable cell lines of Gab2 high expression and Gab2 low expression in two colon carcinoma cell lines.The experiment NOD/SCID mice were divided into 3 groups, 10 rats in each group.The control group included scr/MGC803 inoculation of colon cancer cells, Gab2 reduction group included Gab2 colon cancer cells with reduced inoculation of siGab2/MGC803, and elevated Gab2 group included Gab2/MGC803 colon cancer cells with elevated Gab2 inoculation.Cell migration, tumor size, invasion and cell apoptosis were observed.Results After the treatment, cells transference number calculation results showed that cell migration in GAB2 reduced group was significantly lower than that in GAB2 increased group and control group (P<0.05).NOD/SCID mice were sacrificed 40 days after inoculation, in the comparison of weight of mice before and after inoculation, the Gab2 decreased group was significantly lower than the Gab2 increased group and the control group, and the control group was significantly lower than the Gab2 increased group.In comparison of tumor volume, Gab2 increased group were significantly higher than the Gab2 decreased group and the control group.In Gab2 decreased group, tumor volume inhibition rate was significantly higher than the control group (P<0.05).The correlation analysis showed that MMP-2 and MMP-9 protein expression were positively correlated with GAB2 protein expression.Conclusion Gab2 can effectively regulate the number of cell migration in vitro, significantly inhibit the invasion and metastasis of colon cancer cells in mice, and inhibit the growth and metastasis of colon cancer by regulating the expression of MMP-2 and MMP-9 protein.